Evidence map›Paper›PMID 27473100›Full record

ArticleEndocrine2016

Efficacy of protocols for induction of chronic hyperthyroidism in male and female mice.

Kathrin Engels, Helena Rakov, Denise Zwanziger, Georg Sebastian Hönes, Maren Rehders, Klaudia Brix, Josef Köhrle, Lars Christian Möller, Dagmar Führer

Abstract read
PubMed Publisher
In one paragraph

Article in Endocrine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
2.2field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 26 citations in OpenAlex.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Insight Into Mouse Models of Hyperthyroidism.Frontiers in endocrinology · 2022
    Review
  9. Article
  10. Article
  11. Article
  12. Article
  13. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 1 country.

Kathrin EngelsDepartment of Endocrinology and Metabolism, University Hospital Essen, University Duisburg-Essen, 45122, Essen, Germany.
Helena RakovDepartment of Endocrinology and Metabolism, University Hospital Essen, University Duisburg-Essen, 45122, Essen, Germany.
Denise ZwanzigerDepartment of Endocrinology and Metabolism, University Hospital Essen, University Duisburg-Essen, 45122, Essen, Germany.
Georg Sebastian HönesDepartment of Endocrinology and Metabolism, University Hospital Essen, University Duisburg-Essen, 45122, Essen, Germany.
Maren RehdersDepartment of Life Sciences and Chemistry, Jacobs University Bremen, 28759, Bremen, Germany.
Klaudia BrixDepartment of Life Sciences and Chemistry, Jacobs University Bremen, 28759, Bremen, Germany.
Josef KöhrleInstitut für Experimentelle Endokrinologie, Charité-Universitätsmedizin Berlin, 13353, Berlin, Germany.
Lars Christian MöllerDepartment of Endocrinology and Metabolism, University Hospital Essen, University Duisburg-Essen, 45122, Essen, Germany.
Dagmar FührerDepartment of Endocrinology and Metabolism, University Hospital Essen, University Duisburg-Essen, 45122, Essen, Germany. Dagmar.fuehrer@uk-essen.de.
University of Duisburg-Essen · DEConstructor University · DECharité - Universitätsmedizin Berlin · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Protocols for induction of hyperthyroidism in mice are highly variable and mostly involve short-term thyroid hormone (TH) treatment. In addition, little is known about a possible influence of sex on experimental TH manipulation. Here we analyzed the efficacy of intraperitoneal vs. oral levothyroxine (T4) administration to induce chronic hyperthyroidism in male and female mice and asked which T4 dosing intervals are required to achieve stable organ thyrotoxicosis. T4 was administered intraperitoneally or orally over a period of 6/7 weeks. Assessment included monitoring of body weight, TH serum concentrations, and serial quantitative TH target gene expression analysis in liver and heart. Our results show that both intraperitoneal and oral T4 treatment are reliable methods for induction of chronic hyperthyroidism in mice. Thereby T4 injection intervals should not exceed 48 h and oral levothyroxine should be administered continuously during experiments and up to sacrifice to ensure a hyperthyroid organ state. Furthermore, we found a sex-dependent variation in levothyroxine-induced TH serum state, with significantly higher T4 concentrations in female mice, while expression of investigated classical TH responsive genes in liver and heart did not vary with animal's sex. In summary, our study shows that common approaches for rendering rodents thyrotoxic can also be used for induction of chronic hyperthyroidism in male and female mice. Thereby T4 dosing intervals are critical as are read-out parameters to verify a chronic thyrotoxic organ state.

Indexed as

Disease Models, AnimalThyroxineAnimalsFemaleHyperthyroidismMaleMiceSex FactorsThyroxineHeartLiverSex-differenceTarget genesThyroid hormone actionThyroid hormonesThyrotoxicosis

Identifiers

PMID27473100
OpenAlexW2497776713

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.