ArticlePLoS genetics2016
The Arabidopsis KINβγ Subunit of the SnRK1 Complex Regulates Pollen Hydration on the Stigma by Mediating the Level of Reactive Oxygen Species in Pollen.
Article in PLoS genetics, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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36 citing papers in PubMed, 83 citations in OpenAlex.
- Knockdown of Arabidopsis RUS4 impacts pollen coat formation by affecting flavonoid and lipid metabolism.Planta · 2026Article
- Establishment of fluorescent protein-tagged lines for investigating dynamic localization of organelle and other cell structures in rice pollen tube.Plant methods · 2026Article
- Reactive Oxygen Species Homeostasis Regulates Pistil Development and Pollination inPlants (Basel, Switzerland) · 2026Article
- Characterization and Transcriptome Analysis Reveal Abnormal Pollen Germination in Cytoplasmic Male Sterile Tomato.International journal of molecular sciences · 2025Article
- OsPRK1/2/3-mediated reactive oxygen species signaling is required for pollen tube germination in rice.Journal of integrative plant biology · 2025Article
- Pre-mRNA Splicing Functions in Plant Sexual Reproduction Development.Plants (Basel, Switzerland) · 2025Review
- Molecular mechanisms underlying the negative effects of transient heatwaves on crop fertility.Plant communications · 2024Review
- Pollen viability, longevity, and function in angiosperms: key drivers and prospects for improvement.Plant reproduction · 2024Review
- Article
- Compartmentalization, a key mechanism controlling the multitasking role of the SnRK1 complex.Journal of experimental botany · 2022Review
- Comparative transcriptome analyses revealed key genes involved in high amylopectin biosynthesis in wheat.3 Biotech · 2022Article
- Impaired KIN10 function restores developmental defects in the Arabidopsis trehalose 6-phosphate synthase1 (tps1) mutant.The New phytologist · 2022Article
- MAP3Kε1/2 Interact with MOB1A/1B and Play Important Roles in Control of Pollen Germination through Crosstalk with JA Signaling in Arabidopsis.International journal of molecular sciences · 2022Article
- What are the key mechanisms that alter the morphology of stigmatic papillae inPlant signaling & behavior · 2021Review
- Identification of resurrection genes from the transcriptome of dehydrated and rehydratedPlant signaling & behavior · 2021Article
- A Decade of Pollen Phosphoproteomics.International journal of molecular sciences · 2021Review
- Links between Regulatory Systems of ROS and Carbohydrates in Reproductive Development.Plants (Basel, Switzerland) · 2021Review
- Review
- Reduced expression of a subunit gene of sucrose non-fermenting 1 related kinase, PpSnRK1βγ, confers flat fruit abortion in peach by regulating sugar and starch metabolism.BMC plant biology · 2021Article
- Plant Mitophagy in Comparison to Mammals: What Is Still Missing?International journal of molecular sciences · 2021Review
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pollen-stigma interactions are essential for pollen germination. The highly regulated process of pollen germination includes pollen adhesion, hydration, and germination on the stigma. However, the internal signaling of pollen that regulates pollen-stigma interactions is poorly understood. KINβγ is a plant-specific subunit of the SNF1-related protein kinase 1 complex which plays important roles in the regulation of plant development. Here, we showed that KINβγ was a cytoplasm- and nucleus-localized protein in the vegetative cells of pollen grains in Arabidopsis. The pollen of the Arabidopsis kinβγ mutant could not germinate on stigma, although it germinated normally in vitro. Further analysis revealed the hydration of kinβγ mutant pollen on the stigma was compromised. However, adding water to the stigma promoted the germination of the mutant pollen in vivo, suggesting that the compromised hydration of the mutant pollen led to its defective germination. In kinβγ mutant pollen, the structure of the mitochondria and peroxisomes was destroyed, and their numbers were significantly reduced compared with those in the wild type. Furthermore, we found that the kinβγ mutant exhibited reduced levels of reactive oxygen species (ROS) in pollen. The addition of H2O2 in vitro partially compensated for the reduced water absorption of the mutant pollen, and reducing ROS levels in pollen by overexpressing Arabidopsis CATALASE 3 resulted in compromised hydration of pollen on the stigma. These results indicate that Arabidopsis KINβγ is critical for the regulation of ROS levels by mediating the biogenesis of mitochondria and peroxisomes in pollen, which is required for pollen-stigma interactions during pollination.
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