ArticleCell cycle (Georgetown, Tex.)2016
Elevated intracellular pH appears in aged oocytes and causes oocyte aneuploidy associated with the loss of cohesion in mice.
Article in Cell cycle (Georgetown, Tex.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
- ICSI media buffers: is there room for improvement?Journal of assisted reproduction and genetics · 2026Review
- Hallmarks of female reproductive aging in physiologic aging mice.Nature aging · 2024Review
- Influx of zwitterionic buffer after intracytoplasmic sperm injection (ICSI) membrane piercing alters the transcriptome of human oocytes.Journal of assisted reproduction and genetics · 2024Article
- Knockout of cyclin B1 in granulosa cells causes female subfertility.Cell cycle (Georgetown, Tex.) · 2022Article
- Mouse oocytes carrying metacentric Robertsonian chromosomes have fewer crossover sites and higher aneuploidy rates than oocytes carrying acrocentric chromosomes alone.Scientific reports · 2022Article
- Procyanidin B2 Protects Aged Oocytes Against Meiotic Defects Through Cortical Tension Modulation.Frontiers in veterinary science · 2022Article
- Maturation conditions, post-ovulatory age, medium pH, and ER stress affect [CaJournal of assisted reproduction and genetics · 2021Article
- The Molecular Regulation in the Pathophysiology in Ovarian Aging.Aging and disease · 2021Review
- TRF1 Depletion Reveals Mutual Regulation Between Telomeres, Kinetochores, and Inner Centromeres in Mouse Oocytes.Frontiers in cell and developmental biology · 2021Article
- Need for choosing the ideal pH value for IVF culture media.Journal of assisted reproduction and genetics · 2020Review
- Voltage-gated proton channels exist in the plasma membrane of human oocytes.Human reproduction (Oxford, England) · 2019Article
- Role of WNT signaling in epididymal sperm maturation.Journal of assisted reproduction and genetics · 2018Article
- Merotelic kinetochore attachment in oocyte meiosis II causes sister chromatids segregation errors in aged mice.Cell cycle (Georgetown, Tex.) · 2017Article
- Age-Related Loss of Cohesion: Causes and Effects.International journal of molecular sciences · 2017Review
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Increases in the aneuploidy rate caused by the deterioration of cohesion with increasing maternal age have been well documented. However, the molecular mechanism for the loss of cohesion in aged oocytes remains unknown. In this study, we found that intracellular pH (pHi) was elevated in aged oocytes, which might disturb the structure of the cohesin ring to induce aneuploidy. We observed for the first time that full-grown germinal vesicle (GV) oocytes displayed an increase in pHi with advancing age in CD1 mice. Furthermore, during the in vitro oocyte maturation process, the pHi was maintained at a high level, up to ∼7.6, in 12-month-old mice. Normal pHi is necessary to maintain protein localization and function. Thus, we put forward a hypothesis that the elevated oocyte pHi might be related to the loss of cohesion and the increased aneuploidy in aged mice. Through the in vitro alkalinization treatment of young oocytes, we observed that the increased pHi caused an increase in the aneuploidy rate and the sister inter-kinetochore (iKT) distance associated with the strength of cohesion and caused a decline in the cohesin subunit SMC3 protein level. Young oocytes with elevated pHi exhibited substantially the increase in chromosome misalignment.
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