ArticleVisual neuroscience2016
CACNA1S expression in mouse retina: Novel isoforms and antibody cross-reactivity with GPR179.
Article in Visual neuroscience, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 34 citations in OpenAlex.
- Domain-specific functions of LRIT3 in synaptic assembly and retinal signal transmission.The Journal of biological chemistry · 2026Article
- Exploring the potential for gene therapy in Cav1.4-related retinal channelopathies.Channels (Austin, Tex.) · 2025Review
- LRIT3 expression in cone photoreceptors restores post-synaptic bipolar cell signalplex assembly and partial function iniScience · 2023Article
- Automatic Text-Mining Approach to Identify Molecular Target Candidates Associated with Metabolic Processes for Myotonic Dystrophy Type 1.International journal of environmental research and public health · 2023Article
- Light-dependent changes in the outer plexiform layer of the mouse retina.Frontiers in ophthalmology · 2023Article
- Trophoblast glycoprotein is required for efficient synaptic vesicle exocytosis from retinal rod bipolar cells.Frontiers in cellular neuroscience · 2023Article
- Multiple Calcium Channel Types with Unique Expression Patterns Mediate Retinal Signaling at Bipolar Cell Ribbon Synapses.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Article
- AMIGO1 Promotes Axon Growth and Territory Matching in the Retina.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Article
- A universal protocol for isolating retinal ON bipolar cells across species via fluorescence-activated cell sorting.Molecular therapy. Methods & clinical development · 2021Article
- Synaptic Remodeling in the Cone Pathway After Early Postnatal Horizontal Cell Ablation.Frontiers in cellular neuroscience · 2021Article
- Expression and distribution of trophoblast glycoprotein in the mouse retina.The Journal of comparative neurology · 2020Article
- Skeletal muscle CaPflugers Archiv : European journal of physiology · 2020Review
- Loss of the ER membrane protein complex subunit Emc3 leads to retinal bipolar cell degeneration in aged mice.PloS one · 2020Article
- Presynaptic Expression of LRIT3 Transsynaptically Organizes the Postsynaptic Glutamate Signaling Complex Containing TRPM1.Cell reports · 2019Article
- Reduced expression of theMolecular vision · 2019Article
- Rod Bipolar Cells Require Horizontal Cells for Invagination Into the Terminals of Rod Photoreceptors.Frontiers in cellular neuroscience · 2019Article
- Relevance of tissue specific subunit expression in channelopathies.Neuropharmacology · 2018Review
- Eliminating Glutamatergic Input onto Horizontal Cells Changes the Dynamic Range and Receptive Field Organization of Mouse Retinal Ganglion Cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2018Article
- Homeostatic plasticity shapes the visual system's first synapse.Nature communications · 2017Article
- A missense mutation inJournal of neurophysiology · 2017Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Cacna1s encodes the α1S subunit (Cav1.1) of voltage-dependent calcium channels, and is required for normal skeletal and cardiac muscle function, where it couples with the ryanodine receptor to regulate muscle contraction. Recently CACNA1S was reported to be expressed on the tips of retinal depolarizing bipolar cells (DBCs) and colocalized with metabotropic glutamate receptor 6 (mGluR6), which is critical to DBC signal transduction. Further, in mGluR6 knockout mice, expression at this location is down regulated. We examined RNAseq data from mouse retina and found expression of a novel isoform of Cacna1s. To determine if CACNA1S was a functional component of the DBC signal transduction cascade, we performed immunohistochemistry to visualize its expression in several mouse lines that lack DBC function. Immunohistochemical staining with antibodies to CACNA1S show punctate labeling at the tips of DBCs in wild type (WT) retinas that are absent in Gpr179 nob5 mutant retinas and decreased in Grm6 -/- mouse retinas. CACNA1S and transient receptor potential cation channel, subfamily M, member 1 (TRPM1) staining also colocalized in WT retinas. Western blot analyses for CACNA1S of either retinal lysates or proteins after immunoprecipitation with the CACNA1S antibody failed to show the presence of bands expected for CACNA1S. Mass spectrometric analysis of CACNA1S immunoprecipitated proteins also failed to detect any peptides matching CACNA1S. Immunohistochemistry and western blotting after expression of GPR179 in HEK293T cells indicate that the CACNA1S antibody used here and in the retinal studies published to date, cross-reacts with GPR179. These data suggest caution should be exercised in conferring a role for CACNA1S in DBC signal transduction based solely on immunohistochemical staining.
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