Evidence map›Paper›PMID 27471797›Full record

ArticleRNA biology2016

Structure and target interaction of a G-quadruplex RNA-aptamer.

Kristina Szameit, Katharina Berg, Sven Kruspe, Erica Valentini, Eileen Magbanua, Marcel Kwiatkowski, Isaure Chauvot de Beauchêne, Boris Krichel, Kira Schamoni, Charlotte Uetrecht and 4 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in RNA biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
1.2field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 23 citations in OpenAlex.

  1. Review
  2. An overview of structural approaches to study therapeutic RNAs.Frontiers in molecular biosciences · 2022
    Review
  3. Review
  4. Article
  5. Article
  6. Review
  7. G-quadruplex-based aptamers against protein targets in therapy and diagnostics.Biochimica et biophysica acta. General subjects · 2017
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 6 institutions in 1 country.

Kristina Szameita Institute for Biochemistry and Molecular Biology, Department of Chemistry , University of Hamburg , Hamburg , Germany.
Katharina Berga Institute for Biochemistry and Molecular Biology, Department of Chemistry , University of Hamburg , Hamburg , Germany.
Sven Kruspea Institute for Biochemistry and Molecular Biology, Department of Chemistry , University of Hamburg , Hamburg , Germany.
Erica Valentinib European Molecular Biology Laboratory, Hamburg Unit , Hamburg , Germany.
Eileen Magbanuaa Institute for Biochemistry and Molecular Biology, Department of Chemistry , University of Hamburg , Hamburg , Germany.
Marcel Kwiatkowskic University Medical Center Hamburg-Eppendorf , Department of Clinical Chemistry , Hamburg , Germany.
Isaure Chauvot de Beauchêned Physics Department , Technical University Munich , Garching , Germany.
Boris Krichele Heinrich Pette Institute, Leibniz Institute for Experimental Virology , Hamburg , Germany.
Kira Schamonie Heinrich Pette Institute, Leibniz Institute for Experimental Virology , Hamburg , Germany.
Charlotte Uetrechte Heinrich Pette Institute, Leibniz Institute for Experimental Virology , Hamburg , Germany.
Dmitri I Svergunb European Molecular Biology Laboratory, Hamburg Unit , Hamburg , Germany.
Hartmut Schlüterc University Medical Center Hamburg-Eppendorf , Department of Clinical Chemistry , Hamburg , Germany.
Martin Zachariasd Physics Department , Technical University Munich , Garching , Germany.
Ulrich Hahna Institute for Biochemistry and Molecular Biology, Department of Chemistry , University of Hamburg , Hamburg , Germany.
Universität Hamburg · DEEuropean Molecular Biology Laboratory · DELeibniz Institute of Virology (LIV) · DETechnical University of Munich · DEEuropean X-Ray Free-Electron Laser · DEUniversity Medical Center Hamburg-Eppendorf · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

G-quadruplexes have recently moved into focus of research in nucleic acids, thereby evolving in scientific significance from exceptional secondary structure motifs to complex modulators of gene regulation. Aptamers (nucleic acid based ligands with recognition properties for a specific target) that form Gquadruplexes may have particular potential for therapeutic applications as they combine the characteristics of specific targeting and Gquadruplex mediated stability and regulation. We have investigated the structure and target interaction properties of one such aptamer: AIR-3 and its truncated form AIR-3A. These RNA aptamers are specific for human interleukin-6 receptor (hIL-6R), a key player in inflammatory diseases and cancer, and have recently been exploited for in vitro drug delivery studies. With the aim to resolve the RNA structure, global shape, RNA:protein interaction site and binding stoichiometry, we now investigated AIR-3 and AIR-3A by different methods including RNA structure probing, Small Angle X-ray scattering and microscale thermophoresis. Our findings suggest a broader spectrum of folding species than assumed so far and remarkable tolerance toward different modifications. Mass spectrometry based binding site analysis, supported by molecular modeling and docking studies propose a general Gquadruplex affinity for the target molecule hIL-6R.

Indexed as

AptamersG-quadruplexesinterleukin-6 receptormolecular modelingprotein-RNA interactionSAXS

Identifiers

PMID27471797
PMCPMC5056769
OpenAlexW2494760636

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.