ArticlePlant methods2016
In situ hybridization for the detection of rust fungi in paraffin embedded plant tissue sections.
Article in Plant methods, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 28 citations in OpenAlex.
- Research Progress on Diseases and Pests of Chrysanthemum (2015-2025).International journal of molecular sciences · 2025Review
- StOSD1/UVI4 is crucial for accurate mitotic progression and the maintenance of leaf morphology in potato.BMC plant biology · 2025Article
- Mechanistic Elucidation and Establishment of Drying Kinetic Models of Differential Metabolite Regulation inBiology · 2025Article
- Direct and indirect technical guide for the early detection and management of fungal plant diseases.Current research in microbial sciences · 2024Review
- The effects of different hormone combinations on the growth ofFrontiers in plant science · 2024Article
- Detection of QTLs Regulating Six Agronomic Traits of Rice Based on Chromosome Segment Substitution Lines of Common Wild Rice (Biomolecules · 2022Article
- Histopathology of theFrontiers in plant science · 2021Article
- Chromosome-Scale Reference Genome ofFrontiers in plant science · 2021Article
- Biosynthesis of the starch is improved by the supplement of nickel (NiJournal of plant research · 2020Article
- Recent Advances in Molecular Diagnostics of Fungal Plant Pathogens: A Mini Review.Frontiers in cellular and infection microbiology · 2020Review
- Establishment and optimization of mRNA in situ hybridization system in turnip (Plant methods · 2019Article
- Visualization of the impatiens downy mildew pathogen using fluorescence in situ hybridization (FISH).Plant methods · 2018Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRust fungi are obligate pathogens with multiple life stages often including different spore types and multiple plant hosts. While individual rust pathogens are often associated with specific plants, a wide range of plant species are infected with rust fungi. To study the interactions between these important pathogenic fungi and their host plants, one must be able to differentiate fungal tissue from plant tissue. This can be accomplished using the In situ hybridization (ISH) protocol described here.
resultsTo validate reproducibility using the ISH protocol, samples of Chrysanthemum × morifolium infected with Puccinia horiana, Gladiolus × hortulanus infected with Uromyces transversalis and Glycine max infected with Phakopsora pachyrhizi were tested alongside uninfected leaf tissue samples. The results of these tests show that this technique clearly distinguishes between rust pathogens and their respective host plant tissues.
conclusionsThis ISH protocol is applicable to rust fungi and potentially other plant pathogenic fungi as well. It has been shown here that this protocol can be applied to pathogens from different genera of rust fungi with no background staining of plant tissue. We encourage the use of this protocol for the study of plant pathogenic fungi in paraffin embedded sections of host plant tissue.
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