Evidence map›Paper›PMID 27471254›Full record

ArticleDevelopment (Cambridge, England)2016

SARA regulates neuronal migration during neocortical development through L1 trafficking.

Iván Mestres, Jen-Zen Chuang, Federico Calegari, Cecilia Conde, Ching-Hwa Sung

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Development (Cambridge, England), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.

0numbers the graph read from it
0cells of the map it votes in
9citing papers in PubMed
0.6field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

9 citing papers in PubMed, 12 citations in OpenAlex.

  1. Understanding the Molecular Basis of Miller-Dieker Syndrome.International journal of molecular sciences · 2025
    Review
  2. Review
  3. Article
  4. Article
  5. Tuba Activates Cdc42 during Neuronal Polarization Downstream of the Small GTPase Rab8a.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2021
    Article
  6. Fine-Tuning the TGFβ Signaling Pathway by SARA During Neuronal Development.Frontiers in cell and developmental biology · 2020
    Article
  7. Forces to Drive Neuronal Migration Steps.Frontiers in cell and developmental biology · 2020
    Review
  8. Review
  9. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 3 institutions in 3 countries.

Iván MestresINIMEC, Instituto de Investigación Médica Mercedes y Martín Ferreyra, CONICET, Universidad Nacional de Córdoba UNC, Friuli 2434-5016, Córdoba, Argentina DFG-Research Center for Regenerative Therapies, Cluster of Excellence, TU-Dresden, Fetscherstrasse 105, Dresden 01307, Germany.
Jen-Zen ChuangDepartment of Ophthalmology, Dyson Vision Research Institute, Weill Medical College of Cornell University, New York, NY 10065, USA.
Federico CalegariDFG-Research Center for Regenerative Therapies, Cluster of Excellence, TU-Dresden, Fetscherstrasse 105, Dresden 01307, Germany.
Cecilia CondeINIMEC, Instituto de Investigación Médica Mercedes y Martín Ferreyra, CONICET, Universidad Nacional de Córdoba UNC, Friuli 2434-5016, Córdoba, Argentina Instituto Universitario Ciencias Biomédicas Córdoba (IUCBC), Córdoba 5016, Argentina.
Ching-Hwa SungDepartment of Ophthalmology, Dyson Vision Research Institute, Weill Medical College of Cornell University, New York, NY 10065, USA Departments of Cell and Developmental Biology, Weill Medical College of Cornell University, New York, NY 10065, USA chsung@med.cornell.edu.ORCID 0000-0002-3468-5867
Consejo Nacional de Investigaciones Científicas y Técnicas · ARCornell University · USTechnische Universität Dresden · DE

Funding

MOLECULAR BASIS OF PROTEIN TRANSPORT IN PHOTORECEPTORSR01EY011307 · NEI · WEILL MEDICAL COLL OF CORNELL UNIV · PI SUNG, CHING-HWA · 1996 to 2015
$7.2M
Structural and functional integrity and microenvironment of RPE cellsR01EY016805 · NEI · WEILL MEDICAL COLL OF CORNELL UNIV · PI SUNG, CHING-HWA · 2006 to 2015
$3.5M
NEI NIH HHS R01 EY011307NEI NIH HHS R01 EY016805
6 · The paper itself

Abstract

Emerging evidence suggests that endocytic trafficking of adhesion proteins plays a crucial role in neuronal migration during neocortical development. However, molecular insights into these processes remain elusive. Here, we study the early endosomal protein Smad anchor for receptor activation (SARA) in the developing mouse brain. SARA is enriched at the apical endfeet of radial glia of the neocortex. Although SARA knockdown did not lead to detectable neurogenic phenotypes, SARA-suppressed neurons exhibited impaired orientation and migration across the intermediate zone. Mechanistically, we show that SARA knockdown neurons exhibit increased surface expression of the L1 cell adhesion molecule. Neurons ectopically expressing L1 phenocopy the migration and orientation defects caused by SARA knockdown and display increased contact with neighboring neurites. L1 knockdown effectively rescues SARA suppression-induced phenotypes. SARA knockdown neurons eventually overcome their migration defect and enter later into the cortical plate. Nevertheless, these neurons localize at more superficial cortical layers than their control counterparts. These results suggest that SARA regulates the orientation, multipolar-to-bipolar transition and the positioning of cortical neurons via modulating surface L1 expression.

Indexed as

AnimalsBrainCarrier ProteinsCell LineCell MovementElectroporationFemaleGTP-Binding ProteinsHumansImmunoblottingIntracellular Signaling Peptides and ProteinsMiceNeocortexNeural Cell Adhesion Molecule L1NeurogenesisNeuronsCarrier ProteinsGTP-Binding ProteinsIntracellular Signaling Peptides and ProteinsNeural Cell Adhesion Molecule L1SARA protein, mouseSerine EndopeptidasesZFYVE16 protein, humanAdhesionCortical neuron migrationEndosomal traffickingL1CAMSARAZfyve9

Identifiers

PMID27471254
PMCPMC5047672
OpenAlexW2493557012

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.