Evidence map›Paper›PMID 27471252›Full record

ArticleScience (New York, N.Y.)2016

Div-Seq: Single-nucleus RNA-Seq reveals dynamics of rare adult newborn neurons.

Naomi Habib, Yinqing Li, Matthias Heidenreich, Lukasz Swiech, Inbal Avraham-Davidi, John J Trombetta, Cynthia Hession, Feng Zhang, Aviv Regev

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Science (New York, N.Y.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 347 papers.

0numbers the graph read from it
0cells of the map it votes in
347citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

347 citing papers in PubMed.

  1. Article
  2. Distinct single-nucleus RNA-seq changes among non-neuronal cells in ADNC, LATE-NC, and mixed pathologies.Alzheimer's & dementia : the journal of the Alzheimer's Association · 2026
    Article
  3. Article
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  9. Review
  10. Article
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  12. Single-cell RNA sequencing in stroke and traumatic brain injury: Current achievements, challenges and future perspectives on transcriptomic profiling.Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism · 2026
    Review
  13. Article
  14. Article
  15. Article
  16. Article
  17. Review
  18. Article
  19. Article
  20. Article

287 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Naomi HabibBroad Institute of MIT and Harvard, 415 Main Street, Cambridge, MA 02142, USA. Stanley Center for Psychiatric Research, 75 Ames Street, Cambridge, MA 02142, USA. McGovern Institute of Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Yinqing LiBroad Institute of MIT and Harvard, 415 Main Street, Cambridge, MA 02142, USA. Stanley Center for Psychiatric Research, 75 Ames Street, Cambridge, MA 02142, USA. McGovern Institute of Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Matthias HeidenreichBroad Institute of MIT and Harvard, 415 Main Street, Cambridge, MA 02142, USA. Stanley Center for Psychiatric Research, 75 Ames Street, Cambridge, MA 02142, USA. McGovern Institute of Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Lukasz SwiechBroad Institute of MIT and Harvard, 415 Main Street, Cambridge, MA 02142, USA. Stanley Center for Psychiatric Research, 75 Ames Street, Cambridge, MA 02142, USA. McGovern Institute of Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA.
Inbal Avraham-DavidiBroad Institute of MIT and Harvard, 415 Main Street, Cambridge, MA 02142, USA.
John J TrombettaBroad Institute of MIT and Harvard, 415 Main Street, Cambridge, MA 02142, USA.
Cynthia HessionBroad Institute of MIT and Harvard, 415 Main Street, Cambridge, MA 02142, USA.
Feng ZhangBroad Institute of MIT and Harvard, 415 Main Street, Cambridge, MA 02142, USA. Stanley Center for Psychiatric Research, 75 Ames Street, Cambridge, MA 02142, USA. McGovern Institute of Brain Research, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. Department of Brain and Cognitive Sciences, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. zhang@broadinstitute.org aregev@broadinstitute.org.
Aviv RegevBroad Institute of MIT and Harvard, 415 Main Street, Cambridge, MA 02142, USA. Howard Hughes Medical Institute, Koch Institute of Integrative Cancer Research, Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02139, USA. zhang@broadinstitute.org aregev@broadinstitute.org.

Funding

Pilot & Feasibility ProgramP30DK043351 · NIDDK · MASSACHUSETTS GENERAL HOSPITAL · PI Ramnik J Xavier · 1991 to 2026
$35.3M
Massively-parallel functional interrogation of psychiatric geneticsR01MH110049 · NIMH · BROAD INSTITUTE, INC. · PI ZHANG, FENG · 2015 to 2019
$5.5M
Comprehensive Classification Of Neuronal Subtypes By Single Cell TranscriptomicsU01MH105960 · NIMH · HARVARD UNIVERSITY · PI REGEV, AVIV, SANES, JOSHUA R · 2014 to 2016
$4.5M
Probing Neuropsychiatric Diseases Using Targeted Epigenome and Genome EngineeringDP1MH100706 · NIMH · BROAD INSTITUTE, INC. · PI ZHANG, FENG · 2012 to 2016
$4.3M
LOCATER: Large-scale Observation of Cellular Activity Through Exosomal ReportersR21EY026412 · NEI · BROAD INSTITUTE, INC. · PI ZHANG, FENG · 2015 to 2016
$473k
NEI NIH HHS R21 EY026412NIDDK NIH HHS P30 DK043351NIMH NIH HHS DP1 MH100706NIMH NIH HHS R01 MH110049NIMH NIH HHS U01 MH105960
6 · The paper itself

Abstract

Single-cell RNA sequencing (RNA-Seq) provides rich information about cell types and states. However, it is difficult to capture rare dynamic processes, such as adult neurogenesis, because isolation of rare neurons from adult tissue is challenging and markers for each phase are limited. Here, we develop Div-Seq, which combines scalable single-nucleus RNA-Seq (sNuc-Seq) with pulse labeling of proliferating cells by 5-ethynyl-2'-deoxyuridine (EdU) to profile individual dividing cells. sNuc-Seq and Div-Seq can sensitively identify closely related hippocampal cell types and track transcriptional dynamics of newborn neurons within the adult hippocampal neurogenic niche, respectively. We also apply Div-Seq to identify and profile rare newborn neurons in the adult spinal cord, a noncanonical neurogenic region. sNuc-Seq and Div-Seq open the way for unbiased analysis of diverse complex tissues.

Indexed as

TranscriptomeAnimalsCell DivisionCell NucleusDeoxyuridineHippocampusIsotope LabelingMiceNeurogenesisNeuronsSequence Analysis, RNASingle-Cell AnalysisSpinal CordTranscription, Genetic5-ethynyl-2'-deoxyuridineDeoxyuridine

Identifiers

PMID27471252
PMCPMC5480621

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.