ArticleMicrobial cell factories2016
Rewiring a secondary metabolite pathway towards itaconic acid production in Aspergillus niger.
Article in Microbial cell factories, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 78 citations in OpenAlex.
- Enhancing selective itaconic acid synthesis in Yarrowia lipolytica through targeted metabolite transport reprogramming.Biotechnology for biofuels and bioproducts · 2025Article
- Decoding Pecan's Fungal Foe: A Genomic Insight intoJournal of fungi (Basel, Switzerland) · 2025Article
- Metabolic Engineering of Filamentous FungusJournal of agricultural and food chemistry · 2025Article
- Synthetic biology meets Aspergillus: engineering strategies for next-generation organic acid production.World journal of microbiology & biotechnology · 2025Review
- ReprogrammingScience advances · 2024Article
- Hydroxyhexylitaconic acids as potent IMP-type metallo-β-lactamase inhibitors for controlling carbapenem resistance inMicrobiology spectrum · 2024Article
- Improving pathway prediction accuracy of constraints-based metabolic network models by treating enzymes as microcompartments.Synthetic and systems biotechnology · 2023Article
- Engineering a Phosphoketolase Pathway to Supplement Cytosolic Acetyl-CoA inJournal of fungi (Basel, Switzerland) · 2023Article
- Metabolic engineering using acetate as a promising building block for the production of bio-based chemicals.Engineering microbiology · 2022Review
- Conversion of COProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Synergistic effects on itaconic acid production in engineered Aspergillus niger expressing the two distinct biosynthesis clusters from Aspergillus terreus and Ustilago maydis.Microbial cell factories · 2022Article
- Integration of Aspergillus niger transcriptomic profile with metabolic model identifies potential targets to optimise citric acid production from lignocellulosic hydrolysate.Biotechnology for biofuels and bioproducts · 2022Article
- Genome mining of secondary metabolites from a marine-derived Aspergillus terreus B12.Archives of microbiology · 2021Article
- Beyond the Biosynthetic Gene Cluster Paradigm: Genome-Wide Coexpression Networks Connect Clustered and Unclustered Transcription Factors to Secondary Metabolic Pathways.Microbiology spectrum · 2021Article
- Recent Advances in Biotechnological Itaconic Acid Production, and Application for a Sustainable Approach.Polymers · 2021Review
- Membrane transporters in the bioproduction of organic acids: state of the art and future perspectives for industrial applications.FEMS microbiology letters · 2020Review
- Metabolic engineering of an acid-tolerant yeast strainMetabolic engineering communications · 2020Article
- Metabolic engineering of an industrial3 Biotech · 2020Article
- Biosynthesis of Alkylcitric Acids inFrontiers in microbiology · 2020Article
- Biomass-Derived Production of Itaconic Acid as a Building Block in Specialty Polymers.Polymers · 2019Review
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Authors and funding
7 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe industrially relevant filamentous fungus Aspergillus niger is widely used in industry for its secretion capabilities of enzymes and organic acids. Biotechnologically produced organic acids promise to be an attractive alternative for the chemical industry to replace petrochemicals. Itaconic acid (IA) has been identified as one of the top twelve building block chemicals which have high potential to be produced by biotechnological means. The IA biosynthesis cluster (cadA, mttA and mfsA) has been elucidated in its natural producer Aspergillus terreus and transferred to A. niger to enable IA production. Here we report the rewiring of a secondary metabolite pathway towards further improved IA production through the overexpression of a putative cytosolic citrate synthase citB in a A. niger strain carrying the IA biosynthesis cluster.
resultsWe have previously shown that expression of cadA from A. terreus results in itaconic acid production in A. niger AB1.13, albeit at low levels. This low-level production is boosted fivefold by the overexpression of mttA and mfsA in itaconic acid producing AB1.13 CAD background strains. Controlled batch cultivations with AB1.13 CAD + MFS + MTT strains showed increased production of itaconic acid compared with AB1.13 CAD strain. Moreover, preliminary RNA-Seq analysis of an itaconic acid producing AB1.13 CAD strain has led to the identification of the putative cytosolic citrate synthase citB which was induced in an IA producing strain. We have overexpressed citB in a AB1.13 CAD + MFS + MTT strain and by doing so hypothesize to have targeted itaconic acid production to the cytosolic compartment. By overexpressing citB in AB1.13 CAD + MFS + MTT strains in controlled batch cultivations we have achieved highly increased titers of up to 26.2 g/L IA with a productivity of 0.35 g/L/h while no CA was produced.
conclusionsExpression of the IA biosynthesis cluster in Aspergillus niger AB1.13 strain enables IA production. Moreover, in the AB1.13 CAD strain IA production resulted in overexpression of a putative cytosolic citrate synthase citB. Upon overexpression of citB we have achieved titers of up to 26.2 g/L IA with a productivity of 0.35 g/L/h in controlled batch cultivations. By overexpressing citB we have also diminished side product formation and optimized the production pathway towards IA.
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