ArticleParasitology research2016
Morphological, molecular and MALDI-TOF mass spectrometry identification of ixodid tick species collected in Oromia, Ethiopia.
Article in Parasitology research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 87 citations in OpenAlex.
- Pooled prevalence, species diversity, and risk factors of ixodid ticks in Ethiopian domestic animals: A systematic review and meta-analysis.Current research in parasitology & vector-borne diseases · 2026Review
- "Giant tick" attacks: dynamics of Hyalomma lusitanicum and detection of Rickettsia sibirica mongolitimonae in southern France.Parasite (Paris, France) · 2026Article
- Assessment of MALDI-TOF MS for Arthropod Identification Based on Exuviae Spectra Analysis.Biological procedures online · 2025Article
- Article
- Making MALDI-TOF MS for entomological parameters accessible: A practical guide for in-house library creation.PloS one · 2025Article
- Molecular screening of piroplasms and Anaplasmataceae agents in Hyalomma dromedarii ticks from camels over different seasons in Egypt.Experimental & applied acarology · 2024Article
- Efficiency of MALDI-TOF MS at identifying and discriminating immature stages of cimex lectularius and cimex hemipterus bed bugs.Scientific reports · 2024Article
- Applying MALDI-TOF MS to resolve morphologic and genetic similarities between two Dermacentor tick species of public health importance.Scientific reports · 2024Article
- Seroprevalence ofParasite epidemiology and control · 2024Article
- Forensic parasitology: a new frontier in criminalistics.Forensic sciences research · 2024Review
- First report of dog ticks and tick-borne pathogens they are carrying in Malawi.The Journal of veterinary medical science · 2024Article
- Distribution and prevalence of ixodid tick species (Acari: Ixodidae) infesting cattle in Karamoja region of northeastern Uganda.BMC veterinary research · 2024Article
- Evolution of MALDI-TOF MS Profiles from Lice and Fleas Preserved in Alcohol over Time.Insects · 2023Article
- Distribution and molecular identification of ixodid ticks infesting cattle in Kilombero and Iringa Districts, Tanzania.BMC veterinary research · 2023Article
- MALDI-TOF MS Identification of Dromedary Camel Ticks and Detection of Associated Microorganisms, Southern Algeria.Microorganisms · 2022Article
- Molecular Epidemiological Investigation of Piroplasms and Anaplasmataceae Bacteria in Egyptian Domestic Animals and Associated Ticks.Pathogens (Basel, Switzerland) · 2022Article
- Epidemiological study on Ixodid tick infestation and tick borne haemopathogens on cattle in Awi Zone, northwest Ethiopia.Veterinary medicine and science · 2022Article
- Molecular epidemiology of certain vector-borne bacterial microorganisms in domestic animals and their ectoparasites in Egypt.Tropical animal health and production · 2021Article
- Morphological, molecular and MALDI-TOF MS identification of ticks and tick-associated pathogens in Vietnam.PLoS neglected tropical diseases · 2021Article
- MALDI-TOF mass spectrometry for the identification of freshwater snails from Senegal, including intermediate hosts of schistosomes.PLoS neglected tropical diseases · 2021Article
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Authors and funding
6 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) technology has recently been reported as a promising method for arthropods identification. More recently, our laboratory reported the correct identification of tick species via the MALDI-TOF MS protein spectra profiling of legs from fresh specimens. The aim of the present study was to assess the use of MALDI-TOF MS for correct identification of ixodid tick species preserved in 70 % ethanol during field collection in Ethiopia. Following morphological identification of 12 tick species, the legs from 85 tick specimens were subjected to MALDI-TOF MS. Spectral analysis revealed an intra-species reproducibility and inter-species specificity that were consistent with the morphological classification. To support the results of the MALDI-TOF MS tick species identification, 41 tick specimens comprising 3 to 5 specimens per tick species were used to create a reference spectra database, which was evaluated using the spectra of the 44 remaining tick specimens. The blind tests revealed that 100 % of the tick specimens studied by MALDI-TOF MS were correctly identified. A relevant Log score value (LSV) of >1.8 was recorded for all of the tick species studied by MALDI-TOF MS, except for Rhipicephalus praetextatus. The morphological and MALDI-TOF MS identifications were confirmed by sequencing the 12S ribosomal RNA (rRNA) gene of 40 tick specimens belonging to 11 ixodid species. Taken together, the results of the present study indicate that MALDI-TOF MS is a reliable tool for tick species identification, even after preservation in ethanol, provided that a reference spectra database is built from specimens that represent the respective species stored under the same conditions.
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