ArticleFrontiers in plant science2016
Ascorbate-Deficient vtc2 Mutants in Arabidopsis Do Not Exhibit Decreased Growth.
Article in Frontiers in plant science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 59 citations in OpenAlex.
- Identification of poplar and spruce GDP-mannose pyrophosphorylases involved in mannan and ascorbic acid biosynthesis.Tree physiology · 2026Article
- Simultaneous knockout of VITAMIN C DEFECTIVE 2 and 3 exacerbates ascorbate deficiency and light stress sensitivity.Journal of experimental botany · 2025Article
- Developmental and environmental effects on VTC2-dependent leaf ascorbate accumulation and functions.Journal of experimental botany · 2025Article
- Chloroplastic ascorbate modifies plant metabolism and may act as a metabolite signal regardless of oxidative stress.Plant physiology · 2024Article
- Localization data of the T-DNA insertion site in Arabidopsis line SALK_146824C.Data in brief · 2024Article
- The ascorbate biosynthesis pathway in plants is known, but there is a way to go with understanding control and functions.Journal of experimental botany · 2024Review
- Ascorbic acid metabolism and functions.Journal of experimental botany · 2024Article
- Multi-regulated GDP-l-galactose phosphorylase calls the tune in ascorbate biosynthesis.Journal of experimental botany · 2024Review
- Measurements of Ascorbate and Dehydroascorbate in Plants Using High-Performance Liquid Chromatography.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Organelle-targeted biosensors reveal distinct oxidative events during pattern-triggered immune responses.Plant physiology · 2023Article
- The Functions of Chloroplastic Ascorbate in Vascular Plants and Algae.International journal of molecular sciences · 2023Review
- Comparative Transcriptome Analysis Revealed the Key Genes Regulating Ascorbic Acid Synthesis inInternational journal of molecular sciences · 2021Article
- Distribution and Functions of Monodehydroascorbate Reductases in Plants: Comprehensive Reverse Genetic Analysis ofAntioxidants (Basel, Switzerland) · 2021Article
- Positional-based cloning 'fail-safe' approach is overpowered by wheat chromosome structural variation.The plant genome · 2021Article
- The role of GDP-l-galactose phosphorylase in the control of ascorbate biosynthesis.Plant physiology · 2021Article
- Review
- Dehydroascorbate Reductases and Glutathione Set a Threshold for High-Light-Induced Ascorbate Accumulation.Plant physiology · 2020Article
- Manipulation of Ascorbate Biosynthetic, Recycling, and Regulatory Pathways for Improved Abiotic Stress Tolerance in Plants.International journal of molecular sciences · 2020Review
- Article
- Effect of RiceFrontiers in plant science · 2020Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
In higher plants the L-galactose pathway represents the major route for ascorbate biosynthesis. The first committed step of this pathway is catalyzed by the enzyme GDP-L-galactose phosphorylase and is encoded by two paralogs in Arabidopsis - VITAMIN C2 (VTC2) and VTC5. The first mutant of this enzyme, vtc2-1, isolated via an EMS mutagenesis screen, has approximately 20-30% of wildtype ascorbate levels and has been reported to have decreased growth under standard laboratory conditions. Here, we show that a T-DNA insertion into the VTC2 causes a similar reduction in ascorbate levels, but does not greatly affect plant growth. Subsequent segregation analysis revealed the growth defects of vtc2-1 mutants segregate independently of the vtc2-1 mutation. These observations suggest that it is the presence of an independent cryptic mutation that affects growth of vtc2-1 mutants, and not the 70-80% decrease in ascorbate levels that has been assumed in past studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.