Evidence map›Paper›PMID 27468194›Full record

ArticleWorld journal of gastroenterology2016

Increased duodenal expression of miR-146a and -155 in pediatric Crohn's disease.

Dániel Szűcs, Nóra Judit Béres, Réka Rokonay, Kriszta Boros, Katalin Borka, Zoltán Kiss, András Arató, Attila J Szabó, Ádám Vannay, Erna Sziksz and 2 more

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in World journal of gastroenterology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
21citing papers in PubMed, 1 pooled it
1.5field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

21 citing papers in PubMed, 1 synthesis or guideline pooled it, 29 citations in OpenAlex.

  1. Pooled it
  2. Review
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  6. Article
  7. miRNA Molecules-Late Breaking Treatment for Inflammatory Bowel Diseases?International journal of molecular sciences · 2023
    Review
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  13. Article
  14. Under-expression of microRNA-146a and 21 and their association with Crohn's disease.Indian journal of gastroenterology : official journal of the Indian Society of Gastroenterology · 2020
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 2 institutions in 1 country.

Dániel SzűcsDániel Szűcs, Csaba Bereczki, Department of Pediatrics and Pediatric Health Care Center, University of Szeged, H-6725 Szeged, Hungary.
Nóra Judit BéresDániel Szűcs, Csaba Bereczki, Department of Pediatrics and Pediatric Health Care Center, University of Szeged, H-6725 Szeged, Hungary.
Réka RokonayDániel Szűcs, Csaba Bereczki, Department of Pediatrics and Pediatric Health Care Center, University of Szeged, H-6725 Szeged, Hungary.
Kriszta BorosDániel Szűcs, Csaba Bereczki, Department of Pediatrics and Pediatric Health Care Center, University of Szeged, H-6725 Szeged, Hungary.
Katalin BorkaDániel Szűcs, Csaba Bereczki, Department of Pediatrics and Pediatric Health Care Center, University of Szeged, H-6725 Szeged, Hungary.
Zoltán KissDániel Szűcs, Csaba Bereczki, Department of Pediatrics and Pediatric Health Care Center, University of Szeged, H-6725 Szeged, Hungary.
András AratóDániel Szűcs, Csaba Bereczki, Department of Pediatrics and Pediatric Health Care Center, University of Szeged, H-6725 Szeged, Hungary.
Attila J SzabóDániel Szűcs, Csaba Bereczki, Department of Pediatrics and Pediatric Health Care Center, University of Szeged, H-6725 Szeged, Hungary.
Ádám VannayDániel Szűcs, Csaba Bereczki, Department of Pediatrics and Pediatric Health Care Center, University of Szeged, H-6725 Szeged, Hungary.
Erna SzikszDániel Szűcs, Csaba Bereczki, Department of Pediatrics and Pediatric Health Care Center, University of Szeged, H-6725 Szeged, Hungary.
Csaba BereczkiDániel Szűcs, Csaba Bereczki, Department of Pediatrics and Pediatric Health Care Center, University of Szeged, H-6725 Szeged, Hungary.
Gábor VeresDániel Szűcs, Csaba Bereczki, Department of Pediatrics and Pediatric Health Care Center, University of Szeged, H-6725 Szeged, Hungary.
University of Szeged · HUSemmelweis University · HU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

aimTo evaluate the role of microRNA (miR)-146a, -155 and -122 in the duodenal mucosa of pediatric patients with Crohn's disease (CD) and the effect of transforming growth factor-β (TGF-β) on these miRs in duodenal epithelial and fibroblast cells.

methodsFormalin-fixed, paraffin-embedded biopsies derived from the macroscopically inflamed (CD inflamed: n = 10) and intact (CD intact: n = 10) duodenal mucosa of pediatric CD patients and control children (C: n = 10) were examined. Expression of miR-146a, -155 and -122 was determined by real-time polymerase-chain reaction (PCR). The expression of the above miRs was investigated in recombinant human TGF-β (1 nmol/L, 24 h) or vehicle treated small intestinal epithelial cells (CCL-241) and primary duodenal fibroblast cells derived from healthy children as well.

resultsExpression of miR-146a was significantly higher in the inflamed duodenal mucosa compared to the intact duodenal mucosa of children with CD (CD inflamed: 3.21 ± 0.50 vs CD intact: 0.62 ± 0.26, P ≤ 0.01) and to the control group (CD inflamed: 3.21 ± 0.50 vs C: 1.00 ± 0.33, P ≤ 0.05). The expression of miR-155 was significantly increased in the inflamed region of the duodenum compared to the control group (CD inflamed: 4.87 ± 1.02 vs CONTROL: 1.00 ± 0.40, P ≤ 0.001). The expression of miR-122 was unchanged in the inflamed or intact mucosa of CD patients compared to controls. TGF-β treatment significantly decreased the expression of miR-155 in small intestinal epithelial cells (TGF-β: 0.7 ± 0.083 vs CONTROL: 1 ± 0.09, P ≤ 0.05) and also the expression of miR-146a (TGF-β: 0.67 ± 0.04 vs CONTROL: 1 ± 0.15, P ≤ 0.01) and miR-155 (TGF-β: 0.72 ± 0.09 vs CONTROL: 1 ± 0.06, P ≤ 0.05) in primary duodenal fibroblasts compared to corresponding vehicle treated controls. TGF-β treatment did not influence the expression of miR-122.

conclusionThe elevated expression of miR-146a and -155 in the inflamed duodenal mucosa of CD patients suggests the role of these miRs in the pathomechanism of inflammatory bowel disease. Anti-inflammatory TGF-β plays an important role in the regulation of the expression of these miRs.

Indexed as

AdolescentChildCrohn DiseaseDuodenumEpithelial CellsFemaleFibroblastsHumansIntestinal MucosaIn Vitro TechniquesMaleMicroRNAsReal-Time Polymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionTransforming Growth Factor betaMicroRNAsMIRN122 microRNA, humanMIRN146 microRNA, humanMIRN155 microRNA, humanTransforming Growth Factor betaCrohn’s diseaseInflammatory bowel diseaseMicroRNAsPediatricTransforming growth factor-β

Identifiers

PMID27468194
PMCPMC4948267
OpenAlexW2495783019

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.