ArticlePloS one2016
D-Allulose Production from D-Fructose by Permeabilized Recombinant Cells of Corynebacterium glutamicum Cells Expressing D-Allulose 3-Epimerase Flavonifractor plautii.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
18 citing papers in PubMed, 64 citations in OpenAlex.
- Streamlined production of immobilized D-psicose 3-epimerase via secretion in Pichia pastoris: a new paradigm for industrial D-psicose production.Microbial cell factories · 2025Article
- Recent Advances in the Biosynthesis of Natural Sugar Substitutes in Yeast.Journal of fungi (Basel, Switzerland) · 2023Review
- The Characterization of a Novel D-allulose 3-Epimerase fromFoods (Basel, Switzerland) · 2022Article
- Integrated Whole-Cell Biocatalysis for Trehalose Production from Maltose Using PermeabilizedJournal of microbiology and biotechnology · 2022Article
- Efficient Biosynthesis of 10-Hydroxy-2-decenoic Acid Using a NAD(P)H Regeneration P450 System and Whole-Cell Catalytic Biosynthesis.ACS omega · 2022Article
- Research Advances of d-allulose: An Overview of Physiological Functions, Enzymatic Biotransformation Technologies, and Production Processes.Foods (Basel, Switzerland) · 2021Review
- Characterization of a Recombinant D-Allulose 3-epimerase from Thermoclostridium caenicola with Potential Application in D-Allulose Production.Molecular biotechnology · 2021Article
- D-Allulose 3-epimerase of Bacillus sp. origin manifests profuse heat-stability and noteworthy potential of D-fructose epimerization.Microbial cell factories · 2021Article
- Article
- A Novel d-Allulose 3-Epimerase Gene from the Metagenome of a Thermal Aquatic Habitat and d-Allulose Production by Bacillus subtilis Whole-Cell Catalysis.Applied and environmental microbiology · 2020Article
- Biocatalytic Synthesis of D-Allulose Using Novel D-Tagatose 3-Epimerase FromFrontiers in chemistry · 2020Article
- Review onFrontiers in bioengineering and biotechnology · 2020Review
- The fermentation characteristics of soy yogurt with different content of d-allulose and sucrose fermented by lactic acid bacteria fromFood science and biotechnology · 2019Article
- Redesign of a novel D-allulose 3-epimerase from Staphylococcus aureus for thermostability and efficient biocatalytic production of D-allulose.Microbial cell factories · 2019Article
- Overcoming the thermodynamic equilibrium of an isomerization reaction through oxidoreductive reactions for biotransformation.Nature communications · 2019Article
- Biochemical characterization and biocatalytic application of a novel d-tagatose 3-epimerase fromRSC advances · 2019Article
- Biosensor-based enzyme engineering approach applied to psicose biosynthesis.Synthetic biology (Oxford, England) · 2019Article
- Cell regeneration and cyclic catalysis of engineered Kluyveromyces marxianus of a D-psicose-3-epimerase gene from Agrobacterium tumefaciens for D-allulose production.World journal of microbiology & biotechnology · 2018Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A d-allulose 3-epimerase from Flavonifractor plautii was cloned and expressed in Escherichia coli and Corynebacterium glutamicum. The maximum activity of the enzyme purified from recombinant E. coli cells was observed at pH 7.0, 65°C, and 1 mM Co2+ with a half-life of 40 min at 65°C, Km of 162 mM, and kcat of 25280 1/s. For increased d-allulose production, recombinant C. glutamicum cells were permeabilized via combined treatments with 20 mg/L penicillin and 10% (v/v) toluene. Under optimized conditions, 10 g/L permeabilized cells produced 235 g/L d-allulose from 750 g/L d-fructose after 40 min, with a conversion rate of 31% (w/w) and volumetric productivity of 353 g/L/h, which were 1.4- and 2.1-fold higher than those obtained for nonpermeabilized cells, respectively.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.