ArticlePloS one2016
Proteomic Analysis of the Excretory and Secretory Proteins of Haemonchus contortus (HcESP) Binding to Goat PBMCs In Vivo Revealed Stage-Specific Binding Profiles.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
What it found
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Who cites it
43 citing papers in PubMed, 93 citations in OpenAlex.
- The Lipid Composition of the Exo-Metabolome fromMetabolites · 2025Article
- Partial Protection of Goats againstVaccines · 2024Article
- Nanoparticle containing recombinant excretory/secretory-24 protein ofFrontiers in veterinary science · 2024Article
- Glycan Complexity and Heterogeneity of Glycoproteins in Somatic Extracts and Secretome of the Infective Stage of the Helminth Fasciola hepatica.Molecular & cellular proteomics : MCP · 2023Article
- Metabolite profiling ofFrontiers in cellular and infection microbiology · 2023Article
- Studies on the Requirement of Transthyretin Protein (BxTTR-52) for the Suppression of Host Innate Immunity inInternational journal of molecular sciences · 2022Article
- Functional Diversity of the Excretory/Secretory Proteins of Nematode Parasites.Acta parasitologica · 2022Review
- Identification of Somatic Proteins inHelminthologia · 2022Article
- Recombinant dynein light intermediate chain of Haemonchus contortus affects the functions of goat immune cells in vitro.Parasitology research · 2022Article
- Identification of excretory and secretory proteins from Haemonchus contortus inducing a Th9 immune response in goats.Veterinary research · 2022Article
- Article
- Immunization With RecombinantFrontiers in veterinary science · 2022Article
- Prospects of Using High-Throughput Proteomics to Underpin the Discovery of Animal Host-Nematode Interactions.Pathogens (Basel, Switzerland) · 2021Review
- Fasciola gigantica tegumental calcium-binding EF-hand protein 4 exerts immunomodulatory effects on goat monocytes.Parasites & vectors · 2021Article
- Nanoparticles of Chitosan/Poly(D,L-Lactide-Co-Glycolide) Enhanced the Immune Responses ofInternational journal of nanomedicine · 2021Article
- The GT1-TPS Structural Domain Protein FromFrontiers in immunology · 2021Article
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- Unveiling the Immunomodulatory Characteristics ofAnimals : an open access journal from MDPI · 2020Article
- Advances in the Development of Anti-Vaccines · 2020Review
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Haemonchus contortus is a parasitic gastrointestinal nematode, and its excretory and secretory products (HcESPs) interact extensively with the host cells. In this study, we report the interaction of proteins from HcESPs at different developmental stages to goat peripheral blood mononuclear cells (PBMCs) in vivo using liquid chromatography-tandem mass spectrometry. A total of 407 HcESPs that interacted with goat PBMCs at different time points were identified from a H. contortus protein database using SEQUEST searches. The L4 and L5 stages of H. contortus represented a higher proportion of the identified proteins compared with the early and late adult stages. Both stage-specific interacting proteins and proteins that were common to multiple stages were identified. Forty-seven interacting proteins were shared among all stages. The gene ontology (GO) distributions of the identified goat PBMC-interacting proteins were nearly identical among all developmental stages, with high representation of binding and catalytic activity. Cellular, metabolic and single-organism processes were also annotated as major biological processes, but interestingly, more proteins were annotated as localization processes at the L5 stage than at the L4 and adult stages. Based on the clustering of homologous proteins, we improved the functional annotations of un-annotated proteins identified at different developmental stages. Some unnamed H. contortus ATP-binding cassette proteins, including ADP-ribosylation factor and P-glycoprotein-9, were identified by STRING protein clustering analysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.