ArticlePloS one2016
Prevalence and Genetic Diversity of Giardia duodenalis and Cryptosporidium spp. among School Children in a Rural Area of the Amhara Region, North-West Ethiopia.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers, 1 of them a synthesis that pooled it.
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Who cites it
45 citing papers in PubMed, 1 synthesis or guideline pooled it, 67 citations in OpenAlex.
- Molecular Epidemiology of Giardia Species in Different Hosts in Africa: A Systematic Review and Meta-Analysis.Acta parasitologica · 2026Pooled it
- Examination of ELISA against PCR for assessing treatment efficacy against Cryptosporidium in a clinical trial context.PloS one · 2023Trial
- Risk Factors and Circulating Subtypes ofMicroorganisms · 2025Article
- Genetic diversity ofCurrent research in parasitology & vector-borne diseases · 2025Article
- Prevalence and Genetic Diversity ofJournal of parasitology research · 2025Review
- Asymptomatic carriage of intestinal protists is common in children in Lusaka Province, Zambia.PLoS neglected tropical diseases · 2024Article
- Burden of childhood diarrhea and cholera outbreaks in the Lake Tana Basin (Ethiopia): Review.African health sciences · 2024Review
- Prevalence and associated risk factors of Cryptosporidium infection in calves and hospitalized humans in Libo Kemkem, North Western Ethiopia.Veterinary medicine and science · 2024Article
- Rodent-adaptedOne health (Amsterdam, Netherlands) · 2024Article
- Prevalence and assemblage of Giardia duodenalis in a case-control study of children under 5 years from Jimma, Southwest Ethiopia.Parasitology research · 2023Article
- High Diversity ofTropical medicine and infectious disease · 2023Article
- Review
- Genetic diversity ofJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2022Article
- Neglected Diseases-Parasitic Infections among Slovakian Children from Different Populations and Genotypes ofMicroorganisms · 2022Article
- Cryptosporidium spp. in wild murids (Rodentia) from Corsica, France.Parasitology research · 2022Article
- Selecting a multiplex PCR panel for accurate molecular diagnosis of intestinal protists: a comparative study of AllplexParasite (Paris, France) · 2022Article
- An Update on ZoonoticAnimals : an open access journal from MDPI · 2021Review
- Article
- Detection of enteric parasites and molecular characterization ofParasitology · 2021Article
- Molecular Epidemiology of Human Cryptosporidiosis in Low- and Middle-Income Countries.Clinical microbiology reviews · 2021Review
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Authors and funding
17 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroudGiardia duodenalis and Cryptosporidium spp. are enteric protozoan causing gastrointestinal illness in humans and animals. Giardiasis and cryptosporidiosis are not formally considered as neglected tropical diseases, but belong to the group of poverty-related infectious diseases that impair the development and socio-economic potential of infected individuals in developing countries.
methodsWe report here the prevalence and genetic diversity of G. duodenalis and Cryptosporidium spp. in children attending rural primary schools in the Bahir Dar district of the Amhara Region, Ethiopia. Stool samples were collected from 393 children and analysed by molecular methods. G. duodenalis was detected by real-time PCR, and the assemblages and sub-assemblages were determined by multilocus sequence-based genotyping of the glutamate dehydrogenase and β-giardin genes of the parasite. Detection and identification of Cryptosporidium species was carried out by sequencing of a partial fragment of the small-subunit ribosomal RNA gene. PRINCIPAL
findingsThe PCR-based prevalences of G. duodenalis and Cryptosporidium spp. were 55.0% (216/393) and 4.6% (18/393), respectively. A total of 78 G. duodenalis isolates were successfully characterized, revealing the presence of sub-assemblages AII (10.3%), BIII (28.2%), and BIV (32.0%). Discordant typing results AII/AIII and BIII/BIV were identified in 7.7% and 15.4% of the isolates, respectively. An additional five (6.4%) isolates were assigned to assemblage B. No mixed infections of assemblages A+B were found. Extensive genetic variation at the nucleotide level was observed within assemblage B (but no within assemblage A), resulting in the identification of a large number of sub-types. Cryptosporidium diversity was demonstrated by the occurrence of C. hominis, C. parvum, and C. viatorum in the population under study.
conclusionsOur data suggest an epidemiological scenario with an elevated transmission intensity of a wide range of G. duodenalis genetic variants. Importantly, the elevated degree of genetic diversity observed within assemblage B is consistent with the occurrence of intra-assemblage recombination in G. duodenalis.
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