ArticleOpen biology2016
Structural dissection of a complex Bacteroides ovatus gene locus conferring xyloglucan metabolism in the human gut.
Article in Open biology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
28 citing papers in PubMed, 53 citations in OpenAlex.
- Characterization of glycoside hydrolases involved in xyloglucan degradation in the thermophilic bacterium Thermotoga maritima.Scientific reports · 2025Article
- A functionally augmented carbohydrate utilization locus from herbivore gut microbiota fueled by dietary β-glucans.NPJ biofilms and microbiomes · 2024Article
- The biosynthesis, degradation, and function of cell wall β-xylosylated xyloglucan mirrors that of arabinoxyloglucan.The New phytologist · 2023Article
- Identification and Characterization of Novel Intracellular α-Xylosidase inJournal of applied glycoscience · 2023Article
- The evolutionary advantage of an aromatic clamp in plant family 3 glycoside exo-hydrolases.Nature communications · 2022Article
- Dynamic metabolic interactions and trophic roles of human gut microbes identified using a minimal microbiome exhibiting ecological properties.The ISME journal · 2022Article
- Relandscaping the Gut Microbiota with a Whole Food: Dose-Response Effects to Common Bean.Foods (Basel, Switzerland) · 2022Article
- Polysaccharide utilization loci in Bacteroides determine population fitness and community-level interactions.Cell host & microbe · 2022Article
- Gut microbiome of the largest living rodent harbors unprecedented enzymatic systems to degrade plant polysaccharides.Nature communications · 2022Article
- Characterization of an extracellular α-xylosidase involved in xyloglucan degradation in Aspergillus oryzae.Applied microbiology and biotechnology · 2022Article
- Chemical and nutritional characteristics, and microbial degradation of rapeseed meal recalcitrant carbohydrates: A review.Frontiers in nutrition · 2022Review
- [Research of relationship between frailty and gut microbiota on middle-aged and the aged patients with diabetes].Sheng wu yi xue gong cheng xue za zhi = Journal of biomedical engineering = Shengwu yixue gongchengxue zazhi · 2021Article
- Xyloglucan processing machinery in Xanthomonas pathogens and its role in the transcriptional activation of virulence factors.Nature communications · 2021Article
- Enzymatic degradation of xyloglucans by Aspergillus species: a comparative view of this genus.Applied microbiology and biotechnology · 2021Review
- Modulating the Gut Microbiota of Humans by Dietary Intervention with Plant Glycans.Applied and environmental microbiology · 2021Review
- Unraveling the Metabolic Requirements of the Gut CommensalFrontiers in microbiology · 2021Article
- If you eat it, or secrete it, they will grow: the expanding list of nutrients utilized by human gut bacteria.Journal of bacteriology · 2020Review
- Structure of a GH51 α-L-arabinofuranosidase from Meripilus giganteus: conserved substrate recognition from bacteria to fungi.Acta crystallographica. Section D, Structural biology · 2020Article
- Crystal Structure of α-Xylosidase fromACS sustainable chemistry & engineering · 2020Article
- Oligosaccharides and Complex Carbohydrates: A New Paradigm for Cranberry Bioactivity.Molecules (Basel, Switzerland) · 2020Review
Corrections and comments
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Authors and funding
11 authors at 5 institutions in 4 countries.
Funding
Abstract
The human gastrointestinal tract harbours myriad bacterial species, collectively termed the microbiota, that strongly influence human health. Symbiotic members of our microbiota play a pivotal role in the digestion of complex carbohydrates that are otherwise recalcitrant to assimilation. Indeed, the intrinsic human polysaccharide-degrading enzyme repertoire is limited to various starch-based substrates; more complex polysaccharides demand microbial degradation. Select Bacteroidetes are responsible for the degradation of the ubiquitous vegetable xyloglucans (XyGs), through the concerted action of cohorts of enzymes and glycan-binding proteins encoded by specific xyloglucan utilization loci (XyGULs). Extending recent (meta)genomic, transcriptomic and biochemical analyses, significant questions remain regarding the structural biology of the molecular machinery required for XyG saccharification. Here, we reveal the three-dimensional structures of an α-xylosidase, a β-glucosidase, and two α-l-arabinofuranosidases from the Bacteroides ovatus XyGUL. Aided by bespoke ligand synthesis, our analyses highlight key adaptations in these enzymes that confer individual specificity for xyloglucan side chains and dictate concerted, stepwise disassembly of xyloglucan oligosaccharides. In harness with our recent structural characterization of the vanguard endo-xyloglucanse and cell-surface glycan-binding proteins, the present analysis provides a near-complete structural view of xyloglucan recognition and catalysis by XyGUL proteins.
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