Evidence map›Paper›PMID 27466317›Full record

ArticleMolecular biology of the cell2016

VE-cadherin interacts with cell polarity protein Pals1 to regulate vascular lumen formation.

Benjamin F Brinkmann, Tim Steinbacher, Christian Hartmann, Daniel Kummer, Denise Pajonczyk, Fatemeh Mirzapourshafiyi, Masanori Nakayama, Thomas Weide, Volker Gerke, Klaus Ebnet

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Molecular biology of the cell, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed, 1 pooled it
1.5field-weighted citation impact, top 19% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 1 synthesis or guideline pooled it, 20 citations in OpenAlex.

  1. Pooled it
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  8. Hold Me, but Not Too Tight-Endothelial Cell-Cell Junctions in Angiogenesis.Cold Spring Harbor perspectives in biology · 2018
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Benjamin F BrinkmannInstitute-Associated Research Group "Cell Adhesion and Cell Polarity,", University of Münster, 48419 Münster, Germany Institute of Medical Biochemistry, Center for Molecular Biology of Inflammation, University of Münster, 48419 Münster, Germany Interdisciplinary Clinical Research Center, University of Münster, 48419 Münster, Germany.
Tim SteinbacherInstitute-Associated Research Group "Cell Adhesion and Cell Polarity,", University of Münster, 48419 Münster, Germany Institute of Medical Biochemistry, Center for Molecular Biology of Inflammation, University of Münster, 48419 Münster, Germany.
Christian HartmannInstitute-Associated Research Group "Cell Adhesion and Cell Polarity,", University of Münster, 48419 Münster, Germany Institute of Medical Biochemistry, Center for Molecular Biology of Inflammation, University of Münster, 48419 Münster, Germany Interdisciplinary Clinical Research Center, University of Münster, 48419 Münster, Germany.
Daniel KummerInstitute-Associated Research Group "Cell Adhesion and Cell Polarity,", University of Münster, 48419 Münster, Germany Institute of Medical Biochemistry, Center for Molecular Biology of Inflammation, University of Münster, 48419 Münster, Germany Interdisciplinary Clinical Research Center, University of Münster, 48419 Münster, Germany.
Denise PajonczykInstitute-Associated Research Group "Cell Adhesion and Cell Polarity,", University of Münster, 48419 Münster, Germany Institute of Medical Biochemistry, Center for Molecular Biology of Inflammation, University of Münster, 48419 Münster, Germany.
Fatemeh MirzapourshafiyiLaboratory for Cell Polarity and Organogenesis, Max Planck Institute for Heart and Lung Research, 61231 Bad Nauheim, Germany.
Masanori NakayamaLaboratory for Cell Polarity and Organogenesis, Max Planck Institute for Heart and Lung Research, 61231 Bad Nauheim, Germany.
Thomas WeideDepartment of Internal Medicine D, Division of Molecular Nephrology, University Hospital Münster, Albert-Schweitzer-Campus 1, University of Münster, 48419 Münster, Germany.
Volker GerkeInstitute of Medical Biochemistry, Center for Molecular Biology of Inflammation, University of Münster, 48419 Münster, Germany Cells-in-Motion Cluster of Excellence (EXC 1003-CiM), University of Münster, 48419 Münster, Germany.
Klaus EbnetInstitute-Associated Research Group "Cell Adhesion and Cell Polarity,", University of Münster, 48419 Münster, Germany Institute of Medical Biochemistry, Center for Molecular Biology of Inflammation, University of Münster, 48419 Münster, Germany Interdisciplinary Clinical Research Center, University of Münster, 48419 Münster, Germany ebnetk@uni-muenster.de.
University of Münster · DEMax Planck Institute for Heart and Lung Research · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Blood vessel tubulogenesis requires the formation of stable cell-to-cell contacts and the establishment of apicobasal polarity of vascular endothelial cells. Cell polarity is regulated by highly conserved cell polarity protein complexes such as the Par3-aPKC-Par6 complex and the CRB3-Pals1-PATJ complex, which are expressed by many different cell types and regulate various aspects of cell polarity. Here we describe a functional interaction of VE-cadherin with the cell polarity protein Pals1. Pals1 directly interacts with VE-cadherin through a membrane-proximal motif in the cytoplasmic domain of VE-cadherin. VE-cadherin clusters Pals1 at cell-cell junctions. Mutating the Pals1-binding motif in VE-cadherin abrogates the ability of VE-cadherin to regulate apicobasal polarity and vascular lumen formation. In a similar way, deletion of the Par3-binding motif at the C-terminus of VE-cadherin impairs apicobasal polarity and vascular lumen formation. Our findings indicate that the biological activity of VE-cadherin in regulating endothelial polarity and vascular lumen formation is mediated through its interaction with the two cell polarity proteins Pals1 and Par3.

Indexed as

AnimalsAntigens, CDBinding SitesCadherin 5CadherinsCell LineCell PolarityEndothelial CellsEpithelial CellsHumansIntercellular JunctionsMembrane ProteinsNucleoside-Phosphate KinaseProtein BindingTight JunctionsAntigens, CDCadherin 5CadherinsMembrane ProteinsMPP5 protein, humanNucleoside-Phosphate Kinase

Identifiers

PMID27466317
PMCPMC5025268
OpenAlexW2481503425

What OpenQuestion holds

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LicenceCC BY-NC-SA
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.