ArticleScientific reports2016
Two IIIf Clade-bHLHs from Freesia hybrida Play Divergent Roles in Flavonoid Biosynthesis and Trichome Formation when Ectopically Expressed in Arabidopsis.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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37 citing papers in PubMed, 90 citations in OpenAlex.
- Molecular insights into TT2-type MYB regulators illuminate the complexity of floral flavonoids biosynthesis inHorticulture research · 2025Article
- Regulation of Flavonoid Biosynthesis by the MYB-bHLH-WDR (MBW) Complex in Plants and Its Specific Features in Cereals.International journal of molecular sciences · 2025Review
- Identification of bHLH transcription factors and screening of anthocyanin-related genes in Lagerstroemia indica.Genetica · 2024Article
- Genetic factors explaining anthocyanin pigmentation differences.BMC plant biology · 2024Article
- LaMYC7, a positive regulator of linalool and caryophyllene biosynthesis, confers plant resistance toHorticulture research · 2024Article
- Systematic identification and expression analysis of bHLH gene family reveal their relevance to abiotic stress response and anthocyanin biosynthesis in sweetpotato.BMC plant biology · 2024Article
- Genome-wide identification and expression analysis of bHLH gene family revealed their potential roles in abiotic stress response, anthocyanin biosynthesis and trichome formation inFrontiers in plant science · 2024Article
- Article
- Evolutionary studies of the bHLH transcription factors belonging to MBW complex: their role in seed development.Annals of botany · 2023Review
- Genome-Wide Analysis of the bHLH Gene Family inPlants (Basel, Switzerland) · 2023Article
- The MYB transcription factor RcMYB1 plays a central role in rose anthocyanin biosynthesis.Horticulture research · 2023Article
- Genome-Wide Characterization and Analysis of bHLH Transcription Factors Related to Anthocyanin Biosynthesis inInternational journal of molecular sciences · 2023Article
- Basic Helix-Loop-Helix Transcription Factors: Regulators for Plant Growth Development and Abiotic Stress Responses.International journal of molecular sciences · 2023Review
- Genome-wide identification and expression analysis of the bHLH gene family in cauliflower (Physiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- Comparative Transcriptome Analysis of Purple and Green Non-Heading Chinese Cabbage and Function Analyses ofGenes · 2022Article
- Genome-wide identification and characterization of PdbHLH transcription factors related to anthocyanin biosynthesis in colored-leaf poplar (Populus deltoids).BMC genomics · 2022Article
- Identification and characterization of a novel gene involved in glandular trichome development inFrontiers in plant science · 2022Article
- Transcription factor VvibHLH93 negatively regulates proanthocyanidin biosynthesis in grapevine.Frontiers in plant science · 2022Article
- The bHLH1-DTX35/DFR module regulates pollen fertility by promoting flavonoid biosynthesis inHorticulture research · 2022Article
- Comparative Transcriptome Analysis Revealed Two Alternative Splicing bHLHs Account for Flower Color Alteration in Chrysanthemum.International journal of molecular sciences · 2021Article
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7 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The MBW complex, comprised by R2R3-MYB, basic helix-loop-helix (bHLH) and WD40, is a single regulatory protein complex that drives the evolution of multiple traits such as flavonoid biosynthesis and epidermal cell differentiation in plants. In this study, two IIIf Clade-bHLH regulator genes, FhGL3L and FhTT8L, were isolated and functionally characterized from Freesia hybrida. Different spatio-temporal transcription patterns were observed showing diverse correlation with anthocyanin and proanthocyanidin accumulation. When overexpressed in Arabidopsis, FhGL3L could enhance the anthocyanin accumulation through up-regulating endogenous regulators and late structural genes. Unexpectedly, trichome formation was inhibited associating with the down-regulation of AtGL2. Comparably, only the accumulation of anthocyanins and proanthocyanidins was strengthened in FhTT8L transgenic lines. Furthermore, transient expression assays demonstrated that FhGL3L interacted with AtPAP1, AtTT2 and AtGL1, while FhTT8L only showed interaction with AtPAP1 and AtTT2. In addition, similar activation of the AtDFR promoter was found between AtPAP1-FhGL3L/FhTT8L and AtPAP1- AtGL3/AtTT8 combinations. When FhGL3L was fused with a strong activation domain VP16, it could activate the AtGL2 promoter when co-transfected with AtGL1. Therefore, it can be concluded that the functionality of bHLH factors may have diverged, and a sophisticated interaction and hierarchical network might exist in the regulation of flavonoid biosynthesis and trichome formation.
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