Evidence map›Paper›PMID 27465605›Full record

ArticleJournal of nanobiotechnology2016

Multi-walled carbon nanotubes increase antibody-producing B cells in mice immunized with a tetravalent vaccine candidate for dengue virus.

Luan P Calegari, Roberto S Dias, Michelle D de Oliveira, Carine Ribeiro Pessoa, André S de Oliveira, Ana F C S Oliveira, Cynthia C da Silva, Flavio G Fonseca, Alice F Versiani, Sérgio O De Paula

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of nanobiotechnology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
1.0field-weighted citation impact, top 26% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 14 citations in OpenAlex.

  1. Article
  2. A Comparison Between RecombinantFrontiers in immunology · 2021
    Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Luan P CalegariFederal University of Viçosa, Avenida Peter Henry Rolfs, s/n, Campus Universitário, Viçosa, MG, 36570-900, Brazil.
Roberto S DiasFederal University of Viçosa, Avenida Peter Henry Rolfs, s/n, Campus Universitário, Viçosa, MG, 36570-900, Brazil.
Michelle D de OliveiraFederal University of Viçosa, Avenida Peter Henry Rolfs, s/n, Campus Universitário, Viçosa, MG, 36570-900, Brazil.
Carine Ribeiro PessoaFederal University of Viçosa, Avenida Peter Henry Rolfs, s/n, Campus Universitário, Viçosa, MG, 36570-900, Brazil.
André S de OliveiraFederal Institute of Education, Science and Technology of North of Minas Gerais, R. Gabriel Passos, 259, Centro, Montes Claros, MG, 39400-112, Brazil.
Ana F C S OliveiraFederal Institute of Education, Science and Technology of North of Minas Gerais, R. Gabriel Passos, 259, Centro, Montes Claros, MG, 39400-112, Brazil.
Cynthia C da SilvaFederal University of Viçosa, Avenida Peter Henry Rolfs, s/n, Campus Universitário, Viçosa, MG, 36570-900, Brazil.
Flavio G FonsecaFederal University of Minas Gerais, Belo Horizonte, MG, Brazil.
Alice F VersianiFederal University of Minas Gerais, Belo Horizonte, MG, Brazil.
Sérgio O De PaulaFederal University of Viçosa, Avenida Peter Henry Rolfs, s/n, Campus Universitário, Viçosa, MG, 36570-900, Brazil. depaula@ufv.br.
Universidade Federal de Viçosa · BRUniversidade Federal de Minas Gerais · BR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundIn recent times, studies have demonstrated that carbon nanotubes are good candidates for use as vehicles for transfection of exogenous material into the cells. However, there are few studies evaluating the behavior of carbon nanotubes as DNA vectors and few of these studies have used multi-walled carbon nanotubes (MWCNTs) or carboxylated MWCNTs. Thus, this study aims to assess the MWCNTs' (carboxylated or not) efficiency in the increase in expression of the tetravalent vaccine candidate (TVC) plasmid vector for dengue virus in vitro using Vero cells, and in vivo, through the intramuscular route, to evaluate the immunological response profile.

resultsMulti-walled carbon nanotubes internalized by Vero cells, have been found in the cytoplasm and nucleus associated with the plasmid. However, it was not efficient to increase the messenger ribonucleic acid (mRNA) compared to the pure vaccine candidate associated with Lipofectamine(®) 2000. The in vivo experiments showed that the use of intramuscular injection of the TVC in combination with MWCNTs reduced the immune response compared to pure TVC, in a general way, although an increase was observed in the population of the antibody-producing B cells, as compared to pure TVC.

conclusionsThe results confirm the data found by other authors, which demonstrate the ability of nanotubes to penetrate target cells and reach both the cytoplasm and the cell nucleus. The cytotoxicity values are also in accordance with the literature, which range from 5 to 20 µg/mL. This has been found to be 10 µg/mL in this study. Although the expression levels are higher in cells that receive the pure TVC transfected using Lipofectamine(®) 2000, the nanotubes show an increase in B-cells producing antibodies.

Indexed as

VaccinationAnimalsAntibodies, ViralB-LymphocytesChlorocebus aethiopsDengueDengue VaccinesDengue VirusDisease Models, AnimalFemaleGenetic VectorsHumansLipidsLymphocyte CountMiceMice, Inbred BALB CAntibodies, ViralDengue VaccinesLipidsLipofectamineNanotubes, CarbonDengueIntramuscular routeMulti-walled carbon nanotubesVaccineVero cells

Identifiers

PMID27465605
PMCPMC4964006
OpenAlexW2502926258

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.