ArticleAngewandte Chemie (International ed. in English)2016
Protein Patterns and Oscillations on Lipid Monolayers and in Microdroplets.
Article in Angewandte Chemie (International ed. in English), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed.
- Multimolecular Competition Effect as a Modulator of Protein Localization and Biochemical Networks in Cell-Size Space.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Artificial cell system as a tool for investigating pattern formation mechanisms of intracellular reaction-diffusion waves.Biophysics and physicobiology · 2024Review
- In vitro assembly, positioning and contraction of a division ring in minimal cells.Nature communications · 2022Article
- Min waves without MinC can pattern FtsA-anchored FtsZ filaments on model membranes.Communications biology · 2022Article
- Mode selection mechanism in traveling and standing waves revealed by Min wave reconstituted in artificial cells.Science advances · 2022Article
- Article
- Self-organization Assay for Min Proteins ofBio-protocol · 2020Article
- Regulation of spatiotemporal patterning in artificial cells by a defined protein expression system.Chemical science · 2019Article
- The E. coli MinCDE system in the regulation of protein patterns and gradients.Cellular and molecular life sciences : CMLS · 2019Review
- De novo synthesized Min proteins drive oscillatory liposome deformation and regulate FtsA-FtsZ cytoskeletal patterns.Nature communications · 2019Article
- Article
- Design of Sealable Custom-Shaped Cell Mimicries Based on Self-Assembled Monolayers on CYTOP Polymer.ACS applied materials & interfaces · 2019Article
- Article
- Synthetic cell division via membrane-transforming molecular assemblies.BMC biology · 2019Review
- Beating Vesicles: Encapsulated Protein Oscillations Cause Dynamic Membrane Deformations.Angewandte Chemie (International ed. in English) · 2018Article
- Reverse and forward engineering of protein pattern formation.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Article
- FCS Analysis of Protein Mobility on Lipid Monolayers.Biophysical journal · 2018Article
- Mechanistic insights of the Min oscillator via cell-free reconstitution and imaging.Physical biology · 2018Review
- Sterol transfer, PI4P consumption, and control of membrane lipid order by endogenous OSBP.The EMBO journal · 2017Article
- Artificial cell mimics as simplified models for the study of cell biology.Experimental biology and medicine (Maywood, N.J.) · 2017Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The Min proteins from E.coli position the bacterial cell-division machinery through pole-to-pole oscillations. In vitro, Min protein self-organization can be reconstituted in the presence of a lipid membrane as a catalytic surface. However, Min dynamics have so far not been reconstituted in fully membrane-enclosed volumes. Microdroplets interfaced by lipid monolayers were employed as a simple 3D mimic of cellular compartments to reconstitute Min protein oscillations. We demonstrate that lipid monolayers are sufficient to fulfil the catalytic role of the membrane and thus represent a facile platform to investigate Min protein regulated dynamics of the cell-division protein FtsZ-mts. In particular, we show that droplet containers reveal distinct Min oscillation modes, and reveal a dependence of FtsZ-mts structures on compartment size. Finally, co-reconstitution of Min proteins and FtsZ-mts in droplets yields antagonistic localization, thus demonstrating that droplets indeed support the analysis of complex bacterial self-organization in confined volumes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.