ArticleMicrobial cell factories2016
Enhanced production of polyhydroxybutyrate by multiple dividing E. coli.
Article in Microbial cell factories, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 85 citations in OpenAlex.
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- Demonstration of bioplastic production from CO2 and formate using the reductive glycine pathway in E. coli.PloS one · 2025Article
- Functional loss ofFrontiers in microbiology · 2025Article
- Methanol-based biomanufacturing of fuels and chemicals using native and synthetic methylotrophs.Synthetic and systems biotechnology · 2023Article
- Closing the Gap between Bio-Based and Petroleum-Based Plastic through Bioengineering.Microorganisms · 2022Review
- Review
- Engineering an acetoacetyl-CoA reductase from Cupriavidus necator toward NADH preference under physiological conditions.Scientific reports · 2022Article
- An Eco-Friendly Conversion of Aquaculture Suspended Solid Wastes Into High-Quality Fish Food by Improving Poly-β-Hydroxybutyrate Production.Frontiers in physiology · 2022Article
- Polyhydroxybutyrate (PHB) Production Using an Arabinose-Inducible Expression System in Comparison With Cold Shock Inducible Expression System inFrontiers in bioengineering and biotechnology · 2021Article
- Morphology engineering: a new strategy to construct microbial cell factories.World journal of microbiology & biotechnology · 2020Review
- Comparative analysis of PHAs production bySaudi journal of biological sciences · 2020Article
- Review
- Comparison of Glucose, Acetate and Ethanol as Carbon Resource for Production of Poly(3-Hydroxybutyrate) and Other Acetyl-CoA Derivatives.Frontiers in bioengineering and biotechnology · 2020Article
- Simultaneous Improvements of Pseudomonas Cell Growth and Polyhydroxyalkanoate Production from a Lignin Derivative for Lignin-Consolidated Bioprocessing.Applied and environmental microbiology · 2018Article
- Effects of apolipoprotein E gene polymorphism on the intracellular CaExperimental and therapeutic medicine · 2018Article
- Engineering bacteria for enhanced polyhydroxyalkanoates (PHA) biosynthesis.Synthetic and systems biotechnology · 2017Review
- A novel programmable lysozyme-based lysis system in Pseudomonas putida for biopolymer production.Scientific reports · 2017Article
- Engineering Yarrowia lipolytica for poly-3-hydroxybutyrate production.Journal of industrial microbiology & biotechnology · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMost bacteria are grown in a binary fission way meaning a bacterial cell is equally divided into two. Polyhydroxyalkanoates (PHA) can be accumulated as inclusion bodies by bacteria. The cell division way and morphology have been shown to play an important role in regulating the bacterial growth and PHA storages.
resultsThe common growth pattern of Escherichia coli was changed to multiple fission patterns by deleting fission related genes minC and minD together, allowing the formation of multiple fission rings (Z-rings) in several positions of an elongated cell, thus a bacterial cell was observed to be divided into more than two daughter cells at same time. To further improve cell growth and PHA production, some genes related with division process including ftsQ, ftsL, ftsW, ftsN and ftsZ, together with the cell shape control gene mreB, were all overexpressed in E. coli JM109 ∆minCD. The changing pattern of E. coli cell growth and morphology resulted in more cell dry weights (CDW) and more than 80 % polyhydroxybutyrate (PHB) accumulation increases compared to its binary fission control grown under the same conditions.
conclusionsThis study clearly demonstrated that combined over-expression genes ftsQ, ftsW, ftsN, ftsL and ftsZ together with shape control gene mreB in multiple division bacterial E. coli JM109 ∆minCD benefited PHA accumulation. Our study provides useful information on increasing the yield of PHA by changing the cell division pattern and cell morphology of E. coli.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.