ArticleScientific reports2016
Allele-specific locus binding and genome editing by CRISPR at the p16INK4a locus.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Single-cell and in vivo profiling reveal heterogeneous and organ-specific CRISPR-Cas9 off-target and translocation outcomes.Nature communications · 2026Article
- Quantifying allele-specific CRISPR editing activity with CRISPECTOR2.0.Nucleic acids research · 2024Article
- Chromatin context-dependent effects of epigenetic drugs on CRISPR-Cas9 editing.Nucleic acids research · 2024Article
- Dose-Dependent Effect of Mitochondrial Superoxide Dismutase Gene Overexpression on Radioresistance of HEK293T Cells.International journal of molecular sciences · 2023Article
- Trichostatin A for Efficient CRISPR-Cas9 Gene Editing of Human Pluripotent Stem Cells.The CRISPR journal · 2023Article
- Comprehensive computational analysis of epigenetic descriptors affecting CRISPR-Cas9 off-target activity.BMC genomics · 2022Article
- DNA methylation can alter CRISPR/Cas9 editing frequency and DNA repair outcome in a target-specific manner.The New phytologist · 2022Article
- IL-3-Induced Immediate Expression of c-Cells · 2022Article
- A stem cell marker KLF5 regulates CCAT1 via three-dimensional genome structure in colorectal cancer cells.British journal of cancer · 2022Article
- Protein or ribonucleoprotein-mediated blocking of recombinase polymerase amplification enables the discrimination of nucleotide and epigenetic differences between cell populations.Communications biology · 2021Article
- Radioresistance, DNA Damage and DNA Repair in Cells With Moderate Overexpression of RPA1.Frontiers in genetics · 2020Article
- Allele-specific genome targeting in the development of precision medicine.Theranostics · 2020Review
- Purification of specific DNA species using the CRISPR system.Biology methods & protocols · 2019Review
- Target enrichment from a DNA mixture by oligoribonucleotide interference-PCR (ORNi-PCR).Biology methods & protocols · 2019Article
- Heterochromatin delays CRISPR-Cas9 mutagenesis but does not influence the outcome of mutagenic DNA repair.PLoS biology · 2018Article
- Detection of genome-edited cells by oligoribonucleotide interference-PCR.DNA research : an international journal for rapid publication of reports on genes and genomes · 2018Article
- An enChIP system for the analysis of bacterial genome functions.BMC research notes · 2018Article
- enChIP systems using different CRISPR orthologues and epitope tags.BMC research notes · 2018Article
- Selective disruption of an oncogenic mutant allele by CRISPR/Cas9 induces efficient tumor regression.Nucleic acids research · 2017Article
- Future of rAAV Gene Therapy: Platform for RNAi, Gene Editing, and Beyond.Human gene therapy · 2017Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The clustered regularly interspaced short palindromic repeats (CRISPR) system has been adopted for a wide range of biological applications including genome editing. In some cases, dissection of genome functions requires allele-specific genome editing, but the use of CRISPR for this purpose has not been studied in detail. In this study, using the p16INK4a gene in HCT116 as a model locus, we investigated whether chromatin states, such as CpG methylation, or a single-nucleotide gap form in a target site can be exploited for allele-specific locus binding and genome editing by CRISPR in vivo. First, we showed that allele-specific locus binding and genome editing could be achieved by targeting allele-specific CpG-methylated regions, which was successful for one, but not all guide RNAs. In this regard, molecular basis underlying the success remains elusive at this stage. Next, we demonstrated that an allele-specific single-nucleotide gap form could be employed for allele-specific locus binding and genome editing by CRISPR, although it was important to avoid CRISPR tolerance of a single nucleotide mismatch brought about by mismatched base skipping. Our results provide information that might be useful for applications of CRISPR in studies of allele-specific functions in the genomes.
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