ArticleMicrobial cell factories2016
High production of fatty alcohols in Escherichia coli with fatty acid starvation.
Article in Microbial cell factories, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 55 citations in OpenAlex.
- Engineering Rhodotorula toruloides as a platform organism for de novo synthesis of fatty-acid esters.Nature communications · 2026Article
- Metabolic engineering ofBiodesign research · 2025Article
- Untranslated region engineering strategies for gene overexpression, fine-tuning, and dynamic regulation.Journal of microbiology (Seoul, Korea) · 2025Review
- Metabolic engineering of Corynebacterium glutamicum for fatty alcohol production from glucose and wheat straw hydrolysate.Biotechnology for biofuels and bioproducts · 2023Article
- Metabolic engineering of Rhodotorula toruloides IFO0880 improves C16 and C18 fatty alcohol production from synthetic media.Microbial cell factories · 2022Article
- An Investigation of Stability and Species and Strain-Level Specificity in Bacterial Volatilomes.Frontiers in microbiology · 2021Article
- Opportunities and Challenges for Microbial Synthesis of Fatty Acid-Derived Chemicals (FACs).Frontiers in bioengineering and biotechnology · 2021Review
- Production of Long Chain Fatty Alcohols Found in Bumblebee Pheromones byFrontiers in bioengineering and biotechnology · 2020Article
- Biosynthesis of Fatty Alcohols in Engineered Microbial Cell Factories: Advances and Limitations.Frontiers in bioengineering and biotechnology · 2020Review
- Current Challenges and Opportunities in Non-native Chemical Production by Engineered Yeasts.Frontiers in bioengineering and biotechnology · 2020Review
- Multi-Omics Analysis of Fatty Alcohol Production in Engineered YeastsFrontiers in genetics · 2019Article
- Efficient One-Step Fusion PCR Based on Dual-Asymmetric Primers and Two-Step Annealing.Molecular biotechnology · 2018Article
- Optimization of C16 and C18 fatty alcohol production by an engineered strain of Lipomyces starkeyi.Journal of industrial microbiology & biotechnology · 2018Article
- Microbial synthesis of medium-chain chemicals from renewables.Nature biotechnology · 2017Review
- Modular pathway engineering for the microbial production of branched-chain fatty alcohols.Biotechnology for biofuels · 2017Article
Corrections and comments
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Authors and funding
10 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundMicrobial biofuel synthesis attracting increasing attention. Great advances have been made in producing fatty alcohols from fatty acyl-CoAs and fatty acids in Escherichia coli. However, the low titers and limited knowledge regarding the basic characteristics of fatty alcohols, such as location and toxicity, have hampered large-scale industrialization. Further research is still needed.
resultsIn this study, we designed a novel and efficient strategy to enhance fatty alcohol production by inducing fatty acid starvation. We report the first use of deletions of acyl-ACP thioesterases to enhance fatty alcohol production. Transcriptional analysis was conducted to investigate the mechanism of the designed strategy. Then, fatty alcohol production was further enhanced by deletion of genes from competing pathways. Fatty alcohols were shown to be extracellular products with low toxicity. The final strain, E. coli MGL2, produced fatty alcohols at the remarkable level of 6.33 g/L under fed-batch fermentation, representing the highest reported titer of fatty alcohols produced by microorganisms.
conclusionsDeletions of genes responsible for synthesis of fatty acids and competing products are promising strategies for fatty alcohol production. Our investigation of the location and toxicity of fatty alcohols suggest bright future for fatty alcohol production in E. coli.
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