ArticleScientific reports2016
Fast, label-free super-resolution live-cell imaging using rotating coherent scattering (ROCS) microscopy.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Super-resolution imaging of limited-size objects.Nature photonics · 2026Article
- Enhanced Interferometric Imaging by Rotating Coherent Scattering Microscopy.ACS photonics · 2025Article
- Confocal interferometric scattering microscopy reveals 3D nanoscopic structure and dynamics in live cells.Nature communications · 2023Article
- Label-free optical interferometric microscopy to characterize morphodynamics in living plants.Frontiers in plant science · 2023Review
- Tracing and tracking filamentous structures across scales: A systematic review.Computational and structural biotechnology journal · 2023Review
- Hyperbolic material enhanced scattering nanoscopy for label-free super-resolution imaging.Nature communications · 2022Article
- 100 Hz ROCS microscopy correlated with fluorescence reveals cellular dynamics on different spatiotemporal scales.Nature communications · 2022Article
- Scattering-based Light Microscopy: From Metal Nanoparticles to Single Proteins.Chemical reviews · 2021Review
- Label-free multimodal quantitative imaging flow assay for intrathrombus formation in vitro.Biophysical journal · 2021Article
- Real-Time Measurement of the Hygroscopic Growth Dynamics of Single Aerosol Nanoparticles with Bloch Surface Wave Microscopy.ACS nano · 2020Article
- Fast TIRF-SIM imaging of dynamic, low-fluorescent biological samples.Biomedical optics express · 2020Article
- Super-condenser enables labelfree nanoscopy.Optics express · 2019Article
- Label-free surface-sensitive photonic microscopy with high spatial resolution using azimuthal rotation illumination.Science advances · 2019Article
- Rapid and accurate analysis of stem cell-derived extracellular vesicles with super resolution microscopy and live imaging.Biochimica et biophysica acta. Molecular cell research · 2018Article
- Miniature scanning light-sheet illumination implemented in a conventional microscope.Biomedical optics express · 2018Article
- Dynamics of a Protein Chain Motor Driving Helical Bacteria under Stress.Biophysical journal · 2018Article
- Label-free Imaging and Bending Analysis of Microtubules by ROCS Microscopy and Optical Trapping.Biophysical journal · 2018Article
- Chemically imaging bacteria with super-resolution SERS on ultra-thin silver substrates.Scientific reports · 2017Article
- Super-resolution microscopy with very large working distance by means of distributed aperture illumination.Scientific reports · 2017Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Living cells are highly dynamic systems with cellular structures being often below the optical resolution limit. Super-resolution microscopes, usually based on fluorescence cell labelling, are usually too slow to resolve small, dynamic structures. We present a label-free microscopy technique, which can generate thousands of super-resolved, high contrast images at a frame rate of 100 Hertz and without any post-processing. The technique is based on oblique sample illumination with coherent light, an approach believed to be not applicable in life sciences because of too many interference artefacts. However, by circulating an incident laser beam by 360° during one image acquisition, relevant image information is amplified. By combining total internal reflection illumination with dark-field detection, structures as small as 150 nm become separable through local destructive interferences. The technique images local changes in refractive index through scattered laser light and is applied to living mouse macrophages and helical bacteria revealing unexpected dynamic processes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.