ArticlePLoS pathogens2016
Hyphopodium-Specific VdNoxB/VdPls1-Dependent ROS-Ca2+ Signaling Is Required for Plant Infection by Verticillium dahliae.
Article in PLoS pathogens, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.
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Who cites it
82 citing papers in PubMed, 146 citations in OpenAlex.
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- The ApHSF-ApCtf1β2-ApCUT3 Cascade Couples Host ROS Detoxification to Cuticle Penetration During Fungal Pathogenesis.Molecular plant pathology · 2026Article
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- Exploring the biochemical and biological functions of copper radical oxidases in the vascular wilt phytopathogenApplied and environmental microbiology · 2026Article
- VdPex30, a peroxisomal membrane protein, is involved in carbon metabolism, stress response, and pathogenicity ofMicrobiology spectrum · 2026Article
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- Plant viruses and the microbiome: a complex network shaping plant health and disease resistance.Archives of virology · 2026Review
- Fungal foe: exploring cotton's physiological responses to Verticillium wilt.Frontiers in plant science · 2026Review
- VdOMO contributes to development, stress adaptation, siderophore-associated iron acquisition, and early host colonization inFrontiers in plant science · 2026Article
- Mitochondrial Complex Member VdNuo1 Recruits Superoxide Dismutases VdSOD2/4 to Maintain Superoxide Anion Homeostasis During Pathogenesis in Verticillium dahliae.Molecular plant pathology · 2025Article
- A hypovirulence-associated capsidless bi-segmented ssRNA mycovirus enhances melanin and microsclerotial production in a vascular phytopathogenic fungus.PLoS pathogens · 2025Article
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- The SsSmk1-SsSom1-SsMsb2 Pathway Regulates Infection Cushion Formation and Pathogenicity in Sclerotinia sclerotiorum.Molecular plant pathology · 2025Article
- CWI-MAPKs Regulate the Formation of Hyphopodia Required for Virulence in Ceratocystis fimbriata.Molecular plant pathology · 2025Article
- Roles of NADPH oxidases in regulating redox homeostasis and pathogenesis of the poplar canker fungus Cytospora chrysosperma.Stress biology · 2025Article
- Interaction Between Glycoside Hydrolase FsGH28c fromInternational journal of molecular sciences · 2025Article
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- Review
- Recognition of a Fungal Effector Potentiates Pathogen-Associated Molecular Pattern-Triggered Immunity in Cotton.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
22 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Verticillium dahliae is a phytopathogenic fungus obligate in root infection. A few hyphopodia differentiate from large numbers of hyphae after conidia germination on the root surface for further infection. However, the molecular features and role of hyphopodia in the pathogenicity of V. dahliae remain elusive. In this study, we found that the VdPls1, a tetraspanin, and the VdNoxB, a catalytic subunit of membrane-bound NADPH oxidases for reactive oxygen species (ROS) production, were specifically expressed in hyphopodia. VdPls1 and VdNoxB highly co-localize with the plasma membrane at the base of hyphopodia, where ROS and penetration pegs are generated. Mutant strains, VdΔnoxb and VdΔpls1, in which VdPls1 and VdNoxB were deleted, respectively, developed defective hyphpodia incapable of producing ROS and penetration pegs. Defective plasma membrane localization of VdNoxB in VdΔpls1 demonstrates that VdPls1 functions as an adaptor protein for the recruitment and activation of the VdNoxB. Furthermore, in VdΔnoxb and VdΔpls1, tip-high Ca2+ accumulation was impaired in hyphopodia, but not in vegetative hyphal tips. Moreover, nuclear targeting of VdCrz1 and activation of calcineurin-Crz1 signaling upon hyphopodium induction in wild-type V. dahliae was impaired in both knockout mutants, indicating that VdPls1/VdNoxB-dependent ROS was specifically required for tip-high Ca2+ elevation in hyphopodia to activate the transcription factor VdCrz1 in the regulation of penetration peg formation. Together with the loss of virulence of VdΔnoxb and VdΔpls1, which are unable to initiate colonization in cotton plants, our data demonstrate that VdNoxB/VdPls1-mediated ROS production activates VdCrz1 signaling through Ca2+ elevation in hyphopodia, infectious structures of V. dahliae, to regulate penetration peg formation during the initial colonization of cotton roots.
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