ArticleBiophysical journal2016
Protons Trigger Mitochondrial Flashes.
Article in Biophysical journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 40 citations in OpenAlex.
- Intermittent proton bursts of single lactic acid bacteria.Chemical science · 2024Article
- Aging Disrupts Circadian Rhythms in Mouse Liver Mitochondria.Molecules (Basel, Switzerland) · 2023Review
- Signaling events that occur when cells ofProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Exploring therapeutic potential of mitophagy modulators usingFrontiers in aging neuroscience · 2022Review
- Oxidative bursts of single mitochondria mediate retrograde signaling toward the ER.Molecular cell · 2021Article
- Effects of Metformin on Spontaneous CaInternational journal of molecular sciences · 2021Article
- BacFlash signals acid-resistance gene expression in bacteria.Cell research · 2021Article
- Acute glucose influx-induced mitochondrial hyperpolarization inactivates myosin phosphatase as a novel mechanism of vascular smooth muscle contraction.Cell death & disease · 2021Article
- LETM1: A Single Entity With Diverse Impact on Mitochondrial Metabolism and Cellular Signaling.Frontiers in physiology · 2021Review
- Article
- Mitoflash biogenesis and its role in the autoregulation of mitochondrial proton electrochemical potential.The Journal of general physiology · 2019Article
- Isolating a reverse-mode ATP synthase-dependent mechanism of mitoflash activation.The Journal of general physiology · 2019Article
- Substrate-dependent and cyclophilin D-independent regulation of mitochondrial flashes in skeletal and cardiac muscle.Archives of biochemistry and biophysics · 2019Article
- Article
- Ultrastructural Characterization of Flashing Mitochondria.Contact (Thousand Oaks (Ventura County, Calif.)) · 2018Article
- Overexpressed UCP2 regulates mitochondrial flashes and reverses lipopolysaccharide-induced cardiomyocytes injury.American journal of translational research · 2018Article
- Auto-regulation in the powerhouse.eLife · 2017Article
- Article
- Dendritic mitoflash as a putative signal for stabilizing long-term synaptic plasticity.Nature communications · 2017Article
- Cristae remodeling causes acidification detected by integrated graphene sensor during mitochondrial outer membrane permeabilization.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
14 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Emerging evidence indicates that mitochondrial flashes (mitoflashes) are highly conserved elemental mitochondrial signaling events. However, which signal controls their ignition and how they are integrated with other mitochondrial signals and functions remain elusive. In this study, we aimed to further delineate the signal components of the mitoflash and determine the mitoflash trigger mechanism. Using multiple biosensors and chemical probes as well as label-free autofluorescence, we found that the mitoflash reflects chemical and electrical excitation at the single-organelle level, comprising bursting superoxide production, oxidative redox shift, and matrix alkalinization as well as transient membrane depolarization. Both electroneutral H(+)/K(+) or H(+)/Na(+) antiport and matrix proton uncaging elicited immediate and robust mitoflash responses over a broad dynamic range in cardiomyocytes and HeLa cells. However, charge-uncompensated proton transport, which depolarizes mitochondria, caused the opposite effect, and steady matrix acidification mildly inhibited mitoflashes. Based on a numerical simulation, we estimated a mean proton lifetime of 1.42 ns and diffusion distance of 2.06 nm in the matrix. We conclude that nanodomain protons act as a novel, to our knowledge, trigger of mitoflashes in energized mitochondria. This finding suggests that mitoflash genesis is functionally and mechanistically integrated with mitochondrial energy metabolism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.