ArticleBiophysical journal2016
Spontaneous Formation of a Globally Connected Contractile Network in a Microtubule-Motor System.
Article in Biophysical journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 49 citations in OpenAlex.
- Self-emergent vortex flow of microtubule and kinesin in cell-sized droplets under water/water phase separation.Communications chemistry · 2023Article
- Effects of microtubule length and crowding on active microtubule network organization.iScience · 2023Article
- Cross-linker design determines microtubule network organization by opposing motors.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Shape multistability in flexible tubular crystals through interactions of mobile dislocations.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Extensile to contractile transition in active microtubule-actin composites generates layered asters with programmable lifetimes.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Active beating modes of two clamped filaments driven by molecular motors.Journal of the Royal Society, Interface · 2022Article
- Programmed mechano-chemical coupling in reaction-diffusion active matter.Science advances · 2021Article
- A printable active network actuator built from an engineered biomolecular motor.Nature materials · 2021Article
- Tuning the Properties of Active Microtubule Networks by Depletion Forces.Langmuir : the ACS journal of surfaces and colloids · 2021Article
- Modeling a Microtubule Filaments Mesh Structure from Confocal Microscopy Imaging.Micromachines · 2020Article
- Cytoskeleton polarity is essential in determining orientational order in basal bodies of multi-ciliated cells.PLoS computational biology · 2020Article
- Kinesin-5 Eg5 is essential for spindle assembly and chromosome alignment of mouse spermatocytes.Cell division · 2020Article
- Tunable corrugated patterns in an active nematic sheet.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- From isolated structures to continuous networks: A categorization of cytoskeleton-based motile engineered biological microstructures.Wiley interdisciplinary reviews. Nanomedicine and nanobiotechnology · 2019Review
- Effects of spatial dimensionality and steric interactions on microtubule-motor self-organization.Physical biology · 2019Article
- Microtubule End-Clustering Maintains a Steady-State Spindle Shape.Current biology : CB · 2019Article
- AMPK-dependent phosphorylation of cingulin reversibly regulates its binding to actin filaments and microtubules.Scientific reports · 2018Article
- Article
- Filament rigidity and connectivity tune the deformation modes of active biopolymer networks.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Non-equilibrium assembly of microtubules: from molecules to autonomous chemical robots.Chemical Society reviews · 2017Review
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microtubule (MT) networks play key roles in cell division, intracellular transport, and cell motility. These functions of MT networks occur through interactions between MTs and various associated proteins, notably motor proteins that bundle and slide MTs. Our objective in this study was to address the question of how motors determine the nature of MT networks. We conducted in vitro assays using homotetrameric kinesin Eg5, a motor protein involved in the formation and maintenance of the mitotic spindle. The mixing of Eg5 and MTs produced a range of spatiotemporal dynamics depending on the motor/filament ratio. Low motor/filament ratios produced globally connected static MT networks with sparsely distributed contractile active nodes (motor-accumulating points with radially extending MTs). Increasing the motor/filament ratio facilitated the linking of contractile active nodes and led to a global contraction of the network. When the motor/filament ratio was further increased, densely distributed active nodes formed local clusters and segmented the network into pieces with their strong contractile forces. Altering the properties of the motor through the use of chimeric Eg5, which has kinesin-1 heads, resulted in the generation of many isolated asters. These results suggest that the spatial distribution of contractile active nodes determines the dynamics of MT-motor networks. We then developed a coarse-grained model of MT-motor networks and identified two essential features for reproducing the experimentally observed patterns: an accumulation of motors that form the active nodes necessary to generate contractile forces, and a nonlinear dependency of contractile force on motor densities. Our model also enabled us to characterize the mechanical properties of the contractile network. Our study provides insight into how local motor-MT interactions generate the spatiotemporal dynamics of macroscopic network structures.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.