ArticleCell discovery2016
A genome-scale CRISPR-Cas9 screening method for protein stability reveals novel regulators of Cdc25A.
Article in Cell discovery, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 36 citations in OpenAlex.
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- AMPK phosphorylates and stabilises copper transporter 1 to synergise metformin and copper chelator for breast cancer therapy.British journal of cancer · 2023Article
- Disrupting the phase separation of KAT8-IRF1 diminishes PD-L1 expression and promotes antitumor immunity.Nature cancer · 2023Article
- Targeting the Lysosomal Degradation of Rab22a-NeoF1 Fusion Protein for Osteosarcoma Lung Metastasis.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- SPOP-mediated ubiquitination and degradation of PDK1 suppresses AKT kinase activity and oncogenic functions.Molecular cancer · 2021Article
- DDB1- and CUL4-associated factor 8 plays a critical role in spermatogenesis.Frontiers of medicine · 2021Article
- Targeting the CK1α/CBX4 axis for metastasis in osteosarcoma.Nature communications · 2020Article
- Reporter-Based Screens for the Ubiquitin/Proteasome System.Frontiers in chemistry · 2020Review
- Screening Genes Promoting Exit from Naive Pluripotency Based on Genome-Scale CRISPR-Cas9 Knockout.Stem cells international · 2020Article
- CRISPR Tools for Systematic Studies of RNA Regulation.Cold Spring Harbor perspectives in biology · 2019Review
- NKX2-8 deletion-induced reprogramming of fatty acid metabolism confers chemoresistance in epithelial ovarian cancer.EBioMedicine · 2019Article
- The emerging role for Cullin 4 family of E3 ligases in tumorigenesis.Biochimica et biophysica acta. Reviews on cancer · 2019Review
- CDK16 Phosphorylates and Degrades p53 to Promote Radioresistance and Predicts Prognosis in Lung Cancer.Theranostics · 2018Article
- CRL4BScientific reports · 2017Article
- Protein stability regulators screening assay (Pro-SRSA): protein degradation meets the CRISPR-Cas9 library.Chinese journal of cancer · 2016Article
- Validation of Synthetic CRISPR Reagents as a Tool for Arrayed Functional Genomic Screening.PloS one · 2016Article
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Authors and funding
11 authors at 5 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The regulation of stability is particularly crucial for unstable proteins in cells. However, a convenient and unbiased method of identifying regulators of protein stability remains to be developed. Recently, a genome-scale CRISPR-Cas9 library has been established as a genetic tool to mediate loss-of-function screening. Here, we developed a protein stability regulators screening assay (Pro-SRSA) by combining the whole-genome CRISPR-Cas9 library with a dual-fluorescence-based protein stability reporter and high-throughput sequencing to screen for regulators of protein stability. Using Cdc25A as an example, Cul4B-DDB1(DCAF8) was identified as a new E3 ligase for Cdc25A. Moreover, the acetylation of Cdc25A at lysine 150, which was acetylated by p300/CBP and deacetylated by HDAC3, prevented the ubiquitin-mediated degradation of Cdc25A by the proteasome. This is the first study to report that acetylation, as a novel posttranslational modification, modulates Cdc25A stability, and we suggest that this unbiased CRISPR-Cas9 screening method at the genome scale may be widely used to globally identify regulators of protein stability.
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