ArticleCell discovery2016
The Chp1 chromodomain binds the H3K9me tail and the nucleosome core to assemble heterochromatin.
Article in Cell discovery, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 19 citations in OpenAlex.
- Heterochromatin repeat organization at an individual level: Rex1BD and the 14-3-3 protein coordinate to shape the epigenetic landscape within heterochromatin repeats.BioEssays : news and reviews in molecular, cellular and developmental biology · 2024Review
- Microscale Thermophoresis Analysis of Chromatin Interactions.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Ccr4-Not complex reduces transcription efficiency in heterochromatin.Nucleic acids research · 2022Article
- TASOR epigenetic repressor cooperates with a CNOT1 RNA degradation pathway to repress HIV.Nature communications · 2022Article
- Targeting Epigenetic 'Readers' with Natural Compounds for Cancer Interception.Journal of cancer prevention · 2020Review
- Chasing Tails: Cathepsin-L Improves Structural Analysis of Histones by HX-MS.Molecular & cellular proteomics : MCP · 2019Article
- RNA-induced initiation of transcriptional silencing (RITS) complex structure and function.RNA biology · 2019Review
- Disordered region of H3K9 methyltransferase Clr4 binds the nucleosome and contributes to its activity.Nucleic acids research · 2019Article
- Reading More than Histones: The Prevalence of Nucleic Acid Binding among Reader Domains.Molecules (Basel, Switzerland) · 2018Review
- Cryo-EM of nucleosome core particle interactions in trans.Scientific reports · 2018Article
- Structural rearrangements of the histone octamer translocate DNA.Nature communications · 2018Article
- Histone octamer rearranges to adapt to DNA unwrapping.Nature structural & molecular biology · 2018Article
- Accumulation of RNA on chromatin disrupts heterochromatic silencing.Genome research · 2017Article
- Cryo-electron microscopy of chromatin biology.Acta crystallographica. Section D, Structural biology · 2017Review
- Tailing and degradation of Argonaute-bound small RNAs protect the genome from uncontrolled RNAi.Nature communications · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
To maintain genome stability, cells pack large portions of their genome into silent chromatin or heterochromatin. Histone H3 lysine 9 methylation, a hallmark of heterochromatin, is recognized by conserved readers called chromodomains. But how chromodomains interact with their actual binding partner, the H3K9 methylated nucleosome, remains elusive. We have determined the structure of a nucleosome trimethylated at lysine 9 of histone H3 (H3K9me3 Nucleosome) in a complex with the chromodomain of Chp1, a protein required for RNA interference-dependent heterochromatin formation in fission yeast. The cryo-electron microscopy structure reveals that the chromodomain of Chp1 binds the histone H3 lysine 9 methylated tail and the core of the nucleosome, primarily histones H3 and H2B. Mutations in chromodomain of Chp1 loops, which interact with the nucleosome core, abolished this interaction in vitro. Moreover, fission yeast cells with Chp1 loop mutations have a defect in Chp1 recruitment and heterochromatin formation. This study reveals the structural basis for heterochromatic silencing and suggests that chromodomains could read histone code in the H3 tail and the nucleosome core, which would provide an additional layer of regulation.
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