ArticleFrontiers in plant science2016
Identification of Genomic Insertion and Flanking Sequence of G2-EPSPS and GAT Transgenes in Soybean Using Whole Genome Sequencing Method.
Article in Frontiers in plant science, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 55 citations in OpenAlex.
- T-DNA Analyzer: A Long-Read Sequencing Pipeline for Characterizing T-DNA Insertion Sites in Transgenic Crops.International journal of molecular sciences · 2026Article
- Read-mapping signatures for molecular characterization of transgenic DNA insertions in paired-end Illumina data.BMC biotechnology · 2026Article
- Patch type nucleotide sequence identities between genomes from many different species facilitate illegitimate recombination.Scientific reports · 2026Article
- Unintended effects of exogenous DNA integration in transgenic papaya analyzed at DNA and transcriptional levels.BMC plant biology · 2026Article
- Efficient transgenic maize (Zea Mays L.) detection using low-depth next-generation sequencing.Scientific reports · 2025Article
- Transgene Mapping in Animals: What to Choose?International journal of molecular sciences · 2025Review
- Review of the technology used for structural characterization of the GMO genome using NGS data.Genomics & informatics · 2024Article
- Transgenic poplar (Populus davidiana×P. bolleana Loucne) expressing dsRNA of insect chitinase gene: lines identification and resistance assay.Journal of insect science (Online) · 2024Article
- Technical Note on the quality of DNA sequencing for the molecular characterisation of genetically modified plants.EFSA journal. European Food Safety Authority · 2024Article
- Analysis of the Candidate Genes and Underlying Molecular Mechanism of P198, an RNAi-Related Dwarf and Sterile Line.International journal of molecular sciences · 2023Article
- Molecular Characterization and Efficacy Evaluation of Transgenic Maize HarboringPlants (Basel, Switzerland) · 2023Article
- Review of CRISPR/Cas Systems on Detection of Nucleotide Sequences.Foods (Basel, Switzerland) · 2023Review
- Identification of T-DNA structure and insertion site in transgenic crops using targeted capture sequencing.Frontiers in plant science · 2023Article
- The host niches of soybean rather than genetic modification or glyphosate application drive the assembly of root-associated microbial communities.Microbial biotechnology · 2022Article
- Article
- Development and performance evaluation of whole-genome sequencing with paired-end and mate-pair strategies in molecular characterization of GM crops: One GM rice 114-7-2 line as an example.Food chemistry. Molecular sciences · 2022Article
- A paired-end whole-genome sequencing approach enables comprehensive characterization of transgene integration in rice.Communications biology · 2022Article
- Generation and Identification of the Number of Copies of Exogenous Genes and the T-DNA Insertion Site in SCN-Resistance Transformation Event ZHs1-2.International journal of molecular sciences · 2022Article
- LIFE-Seq: a universal Large Integrated DNA Fragment Enrichment Sequencing strategy for deciphering the transgene integration of genetically modified organisms.Plant biotechnology journal · 2022Article
- Activation of the VQ Motif-Containing Protein GenePlants (Basel, Switzerland) · 2022Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Molecular characterization of sequence flanking exogenous fragment insertion is essential for safety assessment and labeling of genetically modified organism (GMO). In this study, the T-DNA insertion sites and flanking sequences were identified in two newly developed transgenic glyphosate-tolerant soybeans GE-J16 and ZH10-6 based on whole genome sequencing (WGS) method. More than 22.4 Gb sequence data (∼21 × coverage) for each line was generated on Illumina HiSeq 2500 platform. The junction reads mapped to boundaries of T-DNA and flanking sequences in these two events were identified by comparing all sequencing reads with soybean reference genome and sequence of transgenic vector. The putative insertion loci and flanking sequences were further confirmed by PCR amplification, Sanger sequencing, and co-segregation analysis. All these analyses supported that exogenous T-DNA fragments were integrated in positions of Chr19: 50543767-50543792 and Chr17: 7980527-7980541 in these two transgenic lines. Identification of genomic insertion sites of G2-EPSPS and GAT transgenes will facilitate the utilization of their glyphosate-tolerant traits in soybean breeding program. These results also demonstrated that WGS was a cost-effective and rapid method for identifying sites of T-DNA insertions and flanking sequences in soybean.
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