ArticleFrontiers in microbiology2016
Pathophysiological Mechanisms of Staphylococcus Non-aureus Bone and Joint Infection: Interspecies Homogeneity and Specific Behavior of S. pseudintermedius.
Article in Frontiers in microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Staphylococcal internalization into osteoblasts: a partially conserved mechanism across the genus.mBio · 2026Article
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- N-acetyl cysteine as an additive to bone cement against pathogens involved in periprosthetic joint infections.Frontiers in bioengineering and biotechnology · 2025Article
- Integrating multi-wet laboratory diagnostics to study staphylococci in animals in Uganda.BMC microbiology · 2024Article
- Emerging Issues and Initial Insights into Bacterial Biofilms: From Orthopedic Infection to Metabolomics.Antibiotics (Basel, Switzerland) · 2024Review
- Human Colonization and Infection byMicroorganisms · 2023Review
- Invasion of intestinal cells by Staphylococcus warneri, a member of the human gut microbiota.Gut pathogens · 2023Article
- Review on skeletal disorders caused byThe veterinary quarterly · 2022Review
- Bacteriocins Revitalize Non-Effective Penicillin G to Overcome Methicillin-ResistantAntibiotics (Basel, Switzerland) · 2022Article
- Recent Advances in the Control of Clinically Important Biofilms.International journal of molecular sciences · 2022Review
- Transmission of Methicillin-ResistantAntibiotics (Basel, Switzerland) · 2022Article
- From canines to humans: Clinical importance of Staphylococcus pseudintermedius.PLoS pathogens · 2021Article
- Staphylococcus aureus internalization impairs osteoblastic activity and early differentiation process.Scientific reports · 2021Article
- Antibiotic Resistance Profile and Biofilm Production ofPharmaceuticals (Basel, Switzerland) · 2021Article
- Review
- Virulence factors in coagulase-positive staphylococci of veterinary interest other thanThe veterinary quarterly · 2020Review
- Identification and Characterization of Staphylococcus delphini Internalization Pathway in Nonprofessional Phagocytic Cells.Infection and immunity · 2020Article
- Identification of surface proteins in a clinical Staphylococcus haemolyticus isolate by bacterial surface shaving.BMC microbiology · 2020Article
- Bone and Joint Infection InvolvingFrontiers in medicine · 2020Article
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Authors and funding
10 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Implicated in more than 60% of bone and joint infections (BJIs), Staphylococci have a particular tropism for osteoarticular tissue and lead to difficult-to-treat clinical infections. To date, Staphylococcus aureus internalization in non-professional phagocytic cells (NPPCs) is a well-explored virulence mechanism involved in BJI chronicity. Conversely, the pathophysiological pathways associated with Staphylococcus non-aureus (SNA) BJIs have scarcely been studied despite their high prevalence. In this study, 15 reference strains from 15 different SNA species were compared in terms of (i) adhesion to human fibronectin based on adhesion microplate assays and (ii) internalization ability, intracellular persistence and cytotoxicity based on an in vitro infection model using human osteoblasts. Compared to S. aureus, S. pseudintermedius was the only species that significantly adhered to human fibronectin. This species was also associated with high (even superior to S. aureus) internalization ability, intracellular persistence and cytotoxicity. These findings were confirmed using a panel of 17 different S. pseudintermedius isolates. Additionally, S. pseudintermedius internalization by osteoblasts was completely abolished in β1 integrin-deficient murine osteoblasts. These results suggest the involvement of β1 integrin in the invasion process, although this mechanism was previously restricted to S. aureus. In summary, our results suggest that internalization into NPPCs is not a classical pathophysiologic mechanism of SNA BJIs. S. pseudintermedius appears to be an exception, and its ability to invade and subsequently induce cytotoxicity in NPPCs could explain its severe and necrotic forms of infection, notably in dogs, which exhibit a high prevalence of S. pseudintermedius infection.
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