ArticleJournal of veterinary internal medicine2016
Quantitative PCR and Cytology of Bronchoalveolar Lavage Fluid in Dogs with Bordetella bronchiseptica Infection.
Article in Journal of veterinary internal medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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Who cites it
17 citing papers in PubMed, 1 synthesis or guideline pooled it, 31 citations in OpenAlex.
- A systematic review and meta-analyses of the association between 4 mycoplasma species and lower respiratory tract disease in dogs.Journal of veterinary internal medicine · 2019Pooled it
- Evaluation of the IDEXX Procyte Dx Analyzer for the Measurement of Total and Differential Cellular Counts of Bronchoalveolar Lavage Fluid in Healthy Beagles and Dogs with Lower Respiratory Tract Diseases.Veterinary clinical pathology · 2026Article
- Clinical features, risk factors, and outcomes of Bordetella bronchiseptica respiratory infections in dogs diagnosed at a tertiary care institution.Journal of veterinary internal medicine · 2026Article
- Increased neutrophil extracellular traps formation in the bronchoalveolar lavage fluid of dogs with bronchiectasis.Frontiers in veterinary science · 2026Article
- Real-time PCR versus culture of bronchoalveolar lavage fluid for detecting Bordetella bronchiseptica in dogs with persistent lower respiratory signs: a retrospective study of 23 cases.Veterinary research communications · 2025Article
- Proposed treatment algorithms for dogs with chronic bronchitis associated with irreversible airway changes: bronchiectasis and/or bronchomalacia.Frontiers in veterinary science · 2025Article
- Risk factors for canine infectious respiratory disease complex and the pathogens associated with the disease.The Canadian veterinary journal = La revue veterinaire canadienne · 2024Article
- Association between quantitative bacterial culture of bronchoalveolar lavage fluid and antibiotic requirement in dogs with lower respiratory tract signs.Journal of veterinary internal medicine · 2022Article
- Comparison of C-reactive protein concentrations in dogs with Bordetella bronchiseptica infection and aspiration bronchopneumonia.Journal of veterinary internal medicine · 2021Article
- Clinical response to 2 protocols of aerosolized gentamicin in 46 dogs with Bordetella bronchiseptica infection (2012-2018).Journal of veterinary internal medicine · 2020Article
- Aetiology of Canine Infectious Respiratory Disease Complex and Prevalence of its Pathogens in Europe.Journal of comparative pathology · 2020Review
- Analysis of the lung microbiota in dogs with Bordetella bronchiseptica infection and correlation with culture and quantitative polymerase chain reaction.Veterinary research · 2020Article
- Respiratory Dysbiosis in Canine Bacterial Pneumonia: Standard Culture vs. Microbiome Sequencing.Frontiers in veterinary science · 2019Article
- Assessment of real-time PCR cycle threshold values in Microsporum canis culture-positive and culture-negative cats in an animal shelter: a field study.Journal of feline medicine and surgery · 2018Article
- Aspiration techniques for bronchoalveolar lavage in translational respiratory research: Paving the way to develop novel therapeutic moieties.Journal of biological methods · 2017Review
- Mycoplasma cynos-Associated Canine Infectious Respiratory Disease Complex Pneumonia in 13 Dogs.Journal of veterinary emergency and critical care (San Antonio, Tex. : 2001)Article
- The respiratory microbiota and its impact on health and disease in dogs and cats: A One Health perspective.Journal of veterinary internal medicineReview
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe use of quantitative PCR (qPCR) for detection of Bordetella bronchiseptica in bronchoalveolar lavage fluid (BALF) and demonstration of bacteria adhering to ciliated epithelial cells in BALF or bronchial brushing fluid (BBF) has not been assessed in a series of affected dogs. Coinfections can worsen the clinical severity in bordetellosis, but the specific association with Mycoplasma cynos has not been evaluated.
objectivesTo assess the utility of culture, qPCR and cytologic examination of cytospin preparations in the diagnosis of bordetellosis in dogs and the influence of coinfection by M. cynos on disease severity. ANIMALS: Twenty-four referred dogs with B. bronchiseptica infection and 10 healthy dogs.
methodsRetrospective case series. qPCR (B. bronchiseptica and M. cynos) and culture results from BALF were recorded. Cytospin preparations from BALF and BBF were reviewed. qPCR on BALF from 10 healthy dogs were used as negative control.
resultsThe BALF culture and qPCR detected B. bronchiseptica in 14/24 and 18/18 dogs, respectively. Coccobacilli were found adhering to ciliated epithelial cells in 20 of the 21 BALF cytologic preparations where epithelial cells were found, and 2/3 BBF cytologic preparations. Quantitative PCR detected a low level of B. bronchiseptica in one healthy dog. The frequency of detection of M. cynos was not significantly different in B. bronchiseptica (9/17 dogs) compared with healthy dogs (2/10 dogs) (P = .09). CONCLUSION AND CLINICAL IMPORTANCE: Quantitative PCR detection of B. bronchiseptica in BALF appears to be a useful diagnostic tool. Cytologic examination of BALF or BBF, when positive, allows a rapid and reliable diagnosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.