ArticleNature communications2016
A single-molecule view of transcription reveals convoys of RNA polymerases and multi-scale bursting.
Article in Nature communications, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 185 papers.
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Who cites it
185 citing papers in PubMed, 322 citations in OpenAlex.
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- Noninvasive methods to monitor dynamic single-cell events.Trends in cell biology · 2026Review
- Using the simple telegraph model to decipher transcriptional burst regulation across genome-wide data.iScience · 2026Article
- Single-cell transcriptional dynamics in a living vertebrate.Cell systems · 2026Article
- Quantitative RNA spatial profiling using single-molecule RNA FISH on plant tissue cryosections.Plant communications · 2026Article
- Enhancer control of promoter activity and variability via frequency modulation of clustered transcriptional bursts.Nature genetics · 2026Article
- Live-cell imaging of enhancer-promoter dynamics reveals transient contact-driven gene activation.bioRxiv : the preprint server for biology · 2026Article
- Collective RNAP Dynamics Link Transcriptional Strength to Fidelity.The journal of physical chemistry letters · 2026Article
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- From sequence to timescale: a frequency-domain control-theoretic framework linking ncRNA sequence composition to epigenetic regulatory timescales.Frontiers in endocrinology · 2026Article
- What makes genes burst.Trends in cell biology · 2026Review
- RNA polymerase II initiation factors show different dynamic behaviour upon induced transcription in live cells.bioRxiv : the preprint server for biology · 2025Article
- Single-molecule Microscopy Reveals That TFIIE Subunits Dynamically Interact With Preinitiation Complexes in a Manner Impacted by TFIIH.Journal of molecular biology · 2025Article
- Visualizing genetic information flow in space and time.Science China. Life sciences · 2025Review
- Establishment and maintenance of NRT2.1 inter-individual variability in plants.PLoS genetics · 2025Article
- Machine Learning-Driven Prediction of Spatiotemporal Dynamics of Active Nuclei DuringInternational journal of molecular sciences · 2025Article
- Multi-layered dosage compensation of the avian Z chromosome by increased transcriptional burst frequency and elevated translational rates.Nature communications · 2025Article
- A conserved coupling of transcriptional ON and OFF periods underlies bursting dynamics.Nature structural & molecular biology · 2025Article
125 more citing papers are in PubMed but not listed here.
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Authors and funding
14 authors at 1 institution in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Live-cell imaging has revealed unexpected features of gene expression. Here using improved single-molecule RNA microscopy, we show that synthesis of HIV-1 RNA is achieved by groups of closely spaced polymerases, termed convoys, as opposed to single isolated enzymes. Convoys arise by a Mediator-dependent reinitiation mechanism, which generates a transient but rapid succession of polymerases initiating and escaping the promoter. During elongation, polymerases are spaced by few hundred nucleotides, and physical modelling suggests that DNA torsional stress may maintain polymerase spacing. We additionally observe that the HIV-1 promoter displays stochastic fluctuations on two time scales, which we refer to as multi-scale bursting. Each time scale is regulated independently: Mediator controls minute-scale fluctuation (convoys), while TBP-TATA-box interaction controls sub-hour fluctuations (long permissive/non-permissive periods). A cellular promoter also produces polymerase convoys and displays multi-scale bursting. We propose that slow, TBP-dependent fluctuations are important for phenotypic variability of single cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.