ArticleChemical communications (Cambridge, England)2016
Rapid protein immobilization for thin film continuous flow biocatalysis.
Article in Chemical communications (Cambridge, England), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Vortex Fluidic-Mediated Fabrication of Fast Gelated Silica Hydrogels with Embedded Laccase Nanoflowers for Real-Time Biosensing under Flow.ACS applied materials & interfaces · 2020Article
- Three-step-in-one synthesis of supercapacitor MWCNT superparamagnetic magnetite composite material under flow.Nanoscale advances · 2019Article
- Continuous flow biocatalysis.Chemical Society reviews · 2018Review
- Enzymatic synthesis of nucleoside analogues from uridines and vinyl esters in a continuous-flow microreactor.RSC advances · 2018Article
- Article
- Bioengineering toward direct production of immobilized enzymes: A paradigm shift in biocatalyst design.Bioengineered · 2018Review
- The assembly and use of continuous flow systems for chemical synthesis.Nature protocols · 2017Article
- Vortex Fluidic Chemical Transformations.Chemistry (Weinheim an der Bergstrasse, Germany) · 2017Review
- Ten-Minute Protein Purification and Surface Tethering for Continuous-Flow Biocatalysis.Angewandte Chemie (International ed. in English) · 2017Article
- Enzyme Engineering for In Situ Immobilization.Molecules (Basel, Switzerland) · 2016Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
A versatile enzyme immobilization strategy for thin film continuous flow processing is reported. Here, non-covalent and glutaraldehyde bioconjugation are used to immobilize enzymes on the surfaces of borosilicate reactors. This approach requires only ng of protein per reactor tube, with the stock protein solution readily recycled to sequentially coat >10 reactors. Confining reagents to thin films during immobilization reduced the amount of protein, piranha-cleaning solution, and other reagents by ∼96%. Through this technique, there was no loss of catalytic activity over 10 h processing. The results reported here combines the benefits of thin film flow processing with the mild conditions of biocatalysis.
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Registered trials
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