Evidence map›Paper›PMID 27460604›Full record

ArticleInternational journal of obesity (2005)2016

Novel genes on rat chromosome 10 are linked to body fat mass, preadipocyte number and adipocyte size.

A Weingarten, L Turchetti, K Krohn, I Klöting, M Kern, P Kovacs, M Stumvoll, M Blüher, N Klöting

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Article in International journal of obesity (2005), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.9field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 7 citations in OpenAlex.

  1. Article
  2. Article
  3. Transglutaminases and Obesity in Humans: Association ofInternational journal of molecular sciences · 2020
    Article
  4. Rat Models of Metabolic Syndrome.Methods in molecular biology (Clifton, N.J.) · 2019
    Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 4 institutions in 1 country.

A WeingartenIFB AdiposityDiseases, University of Leipzig, Leipzig, Germany.
L TurchettiDepartment of Medicine, University of Leipzig, Leipzig, Germany.
K KrohnCore Unit IZKF, University of Leipzig, Leipzig, Germany.
I KlötingLaboratory Animal Science, University of Greifswald, Karlsburg, Germany.
M KernIFB AdiposityDiseases, University of Leipzig, Leipzig, Germany.
P KovacsIFB AdiposityDiseases, University of Leipzig, Leipzig, Germany.
M StumvollIFB AdiposityDiseases, University of Leipzig, Leipzig, Germany.
M BlüherIFB AdiposityDiseases, University of Leipzig, Leipzig, Germany.
N KlötingIFB AdiposityDiseases, University of Leipzig, Leipzig, Germany.
IFB Adiposity Diseases · DELeipzig University · DEUniversität Greifswald · DEHeinrich Heine University Düsseldorf · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundThe genetic architecture of obesity is multifactorial. We have previously identified a quantitative trait locus (QTL) on rat chromosome 10 in a F2 cross of Wistar Ottawa Karlsburg (WOKW) and Dark Agouti (DA) rats responsible for obesity-related traits. The QTL was confirmed in congenic DA.WOKW10 rats. To pinpoint the region carrying causal genes, we established two new subcongenic lines, L1 and L2, with smaller refined segments of chromosome 10 to identify novel candidate genes.

methodsAll lines were extensively characterized under different diet conditions. We employed transcriptome analysis in visceral adipose tissue (VAT) by RNA-Seq technology to identify potential underlying genes in the segregating regions. Three candidate genes were measured in human paired samples of VAT and subcutaneous (SC) AT (SAT) (N=304) individuals with a wide range of body weight and glucose homeostasis parameters.

resultsDA.WOKW and L1 subcongenic lines were protected against body fat gain under high-fat diet (HFD), whereas L2 and DA had significantly more body fat after high-fat feeding. Interestingly, adipocyte size distribution in SAT and epigonadal AT of L1 subcongenic rats did not undergo typical ballooning under HFD and the number of preadipocytes in AT was significantly elevated in L2 compared with L1 and parental rats. Transcriptome analysis identified three candidate genes in VAT on rat chromosome 10. In humans, these candidate genes were differentially expressed between SAT and VAT. Moreover, HID1 mRNA significantly correlates with parameters of obesity and glucose metabolism.

conclusionsOur data suggest novel candidate genes for obesity that map on rat chromosome 10 in an interval 102.2-104.7 Mb and are strongly associated with body fat mass regulation, preadipocyte number and adipocyte size in rats. Among those genes, AT head involution defective (HID1) mRNA expression may be relevant for human fat distribution and glucose homeostasis.

Indexed as

AdipocytesAdipose TissueAnimalsCell CountCell SizeChromosomes, MammalianDisease Models, AnimalFemaleGene Expression ProfilingGenetic TechniquesHumansIntercellular Signaling Peptides and ProteinsMaleMembrane ProteinsMiceMice, Inbred C57BLDlk1 protein, ratIntercellular Signaling Peptides and ProteinsMembrane Proteins

Identifiers

PMID27460604
OpenAlexW2481264448

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.