ArticleCurrent microbiology2016
Purification and Evaluation of Polysaccharide Intercellular Adhesion (PIA) Antigen from Staphylococcus epidermidis.
Article in Current microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 20 citations in OpenAlex.
- Comparison of the antimicrobial, anti-biofilm, and cytotoxic effects of thymol/ampicillin and thymol/cefotaxime against Escherichia coli bacteria.BMC research notes · 2026Article
- Investigation of the effect of anti-PIA/PNAG antibodies on biofilm formation inFrontiers in microbiology · 2025Article
- Multitasking functions of bacterial extracellular DNA in biofilms.Journal of bacteriology · 2024Review
- Analysis of immunogenicity and purification methods in conjugated polysaccharide vaccines: a new approach in fighting pathogenic bacteria.Frontiers in immunology · 2024Review
- The frequency of adherence, biofilm-associated, Arginine Catabolic Mobile element genes, and biofilm formation in clinical and healthcare worker coagulase-negative staphylococci isolates.BMC microbiology · 2023Article
- A General Map of Transcriptional Expression of Virulence, Metabolism, and Biofilm Formation Adaptive Changes ofFrontiers in microbiology · 2022Article
- Evaluation of the Negative Predictive Value of Methicillin-ResistantInfection and drug resistance · 2022Article
- Multi-epitope chimeric vaccine designing and novel drug targets prioritization against multi-drug resistantFrontiers in microbiology · 2022Article
- Genetic Diversity, Antibiotic Resistance, and Virulence Gene Features of Methicillin-ResistantInfection and drug resistance · 2022Article
- PIA and rSesC Mixture Arisen Antibodies Could Inhibit the Biofilm-Formation inReports of biochemistry & molecular biology · 2021Article
- Methicillin- and Vancomycin-ResistantFrontiers in microbiology · 2021Article
- Purification of PIA and rSesC as Putative Vaccine Candidates AgainstReports of biochemistry & molecular biology · 2019Article
- Determining the Biofilm Forming Gene Profile ofReports of biochemistry & molecular biology · 2019Article
- Construction and analysis of alginate-based honey hydrogel as an ointment to heal of rat burn wound related infections.International journal of burns and trauma · 2018Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The polysaccharide intercellular adhesin (PIA) confers major functional effects in biofilm formation, which bears an important role in the pathogenicity of Staphylococcus epidermidis. Following the identification of biofilm-forming strains by biochemical and molecular methods, isogenic strain was prepared and in vitro biofilm formation assay was performed consequently. By parallel analysis of both the PIA-positive and PIA-negative strains using size exclusion chromatography by Fast protein liquid chromatography (FPLC) method, the respective PIA was purified. Recovered PIA was examined using colorimetric and hemagglutination assays. Finally, the recovered PIA was analyzed using Fourier-transform infrared spectroscopy and proton nuclear magnetic resonance spectroscopy methods. By the parallel purification process and comparison of the obtained graphs from the FPLC detector, fractions near the void volume were determined as PIA. The colorimetric and hemagglutination assays were applied and the content of carbohydrates (hexose = 620 µg/ml, hexosamine = 5700 µg/ml and ketoses = 170 µg/ml) and hemagglutination titer (1:128) in recovered polysaccharide were determined. This study shows that PIA has a significant role in the biofilm formation in S. epidermidis strains. The recovered polysaccharide and its molecular weight were analyzed within the near void volume of the utilized column.
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