Evidence map›Paper›PMID 27460583›Full record

ArticleCurrent microbiology2016

Characterization of Ciprofloxacin-Resistant and Ciprofloxacin-Susceptible Uropathogenic Escherichia coli Obtained from Patients with Gynecological Cancer.

Muniqui S Capett, Patricia Vollú-Silva, Vanessa A Melchiades, Luciana C Bokehi, Fernanda M Araújo, Ianick Souto Martins, Felipe P G Neves, Alice G M Gonzalez, Eric Oswald, Geraldo R de Paula and 1 more

Abstract read
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In one paragraph

Article in Current microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.3field-weighted citation impact, top 42% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 4 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 2 countries.

Muniqui S CapettDepartamento de Tecnologia Farmacêutica, Faculdade de Farmácia, Universidade Federal Fluminense, Rua Mário Viana 523, Santa Rosa, Niterói, RJ, 24241-002, Brazil.
Patricia Vollú-SilvaDepartamento de Tecnologia Farmacêutica, Faculdade de Farmácia, Universidade Federal Fluminense, Rua Mário Viana 523, Santa Rosa, Niterói, RJ, 24241-002, Brazil.
Vanessa A MelchiadesDepartamento de Tecnologia Farmacêutica, Faculdade de Farmácia, Universidade Federal Fluminense, Rua Mário Viana 523, Santa Rosa, Niterói, RJ, 24241-002, Brazil.
Luciana C BokehiDepartamento de Tecnologia Farmacêutica, Faculdade de Farmácia, Universidade Federal Fluminense, Rua Mário Viana 523, Santa Rosa, Niterói, RJ, 24241-002, Brazil.
Fernanda M AraújoDepartamento de Tecnologia Farmacêutica, Faculdade de Farmácia, Universidade Federal Fluminense, Rua Mário Viana 523, Santa Rosa, Niterói, RJ, 24241-002, Brazil.
Ianick Souto MartinsFaculdade de Medicina, Universidade Federal Fluminense, Niterói, RJ, Brazil.
Felipe P G NevesInstituto Biomédico, Universidade Federal Fluminense, Niterói, RJ, Brazil.
Alice G M GonzalezDepartamento de Tecnologia Farmacêutica, Faculdade de Farmácia, Universidade Federal Fluminense, Rua Mário Viana 523, Santa Rosa, Niterói, RJ, 24241-002, Brazil.
Eric OswaldInstitut de Recherche en Santé Digestive, INSERM UMR1220, Toulouse, France.
Geraldo R de PaulaDepartamento de Tecnologia Farmacêutica, Faculdade de Farmácia, Universidade Federal Fluminense, Rua Mário Viana 523, Santa Rosa, Niterói, RJ, 24241-002, Brazil.
Lenise A TeixeiraDepartamento de Tecnologia Farmacêutica, Faculdade de Farmácia, Universidade Federal Fluminense, Rua Mário Viana 523, Santa Rosa, Niterói, RJ, 24241-002, Brazil. tlenise@hotmail.com.
Universidade Federal Fluminense · BRDigestive Health Research Institute · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The objective of this work was to assess the genetic characteristics of uropathogenic Escherichia coli, ciprofloxacin resistance or susceptibility, obtained from patients with gynecological cancer and urinary tract infection (UTI). Seventy-seven E. coli ciprofloxacin-resistant isolates and 38 ciprofloxacin-susceptible were analyzed by polymerase chain reaction (PCR) to determine the phylogenetic groups, virulence factors as iucC, fyuA, hlyC, cnf1 genes, and pks pathogenicity island. The presence of genes related to ciprofloxacin resistance such as qnrA, qnrB, qnrS, aac(6')-Ib-cr, and qepA, and the sequencing of DNA gyrase genes and topoisomerase IV were determined. The genetic profile of the isolates was determined by pulsed-field gel electrophoresis (PFGE). Statistical analysis was performed using Fisher's exact test and Chi-square test. Phylogenetic group B2 was the most prevalent although a great genetic diversity was observed by PFGE. Only genes associated to siderophores were found in ciprofloxacin-resistant isolates; however, in ciprofloxacin-susceptible isolates, genes related to siderophores and toxin, were detected. Additionally qnrB was detected in both populations, ciprofloxacin resistant and susceptible. DNA mutations in gyrA were Ser-83-Leu and Asp-87-Asn and in parC were Ser-80-Ile and Glu-84-Val, Glu-84-Lys. In conclusion, it was observed a high prevalence of qnrB in the population studied; in addition, it was the first time the pks island was observed only in ciprofloxacin-susceptible isolates.

Indexed as

Drug Resistance, BacterialAnti-Bacterial AgentsCiprofloxacinEscherichia coli InfectionsEscherichia coli ProteinsFemaleGenital Neoplasms, FemaleHumansMicrobial Sensitivity TestsPhylogenyUrinary Tract InfectionsUropathogenic Escherichia coliAnti-Bacterial AgentsCiprofloxacinEscherichia coli Proteins

Identifiers

PMID27460583
OpenAlexW2499016875

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.