ArticleScientific reports2016
Rapid identification of intact bacterial resistance plasmids via optical mapping of single DNA molecules.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Strain-Level Typing ofACS infectious diseases · 2025Article
- Comparison of CTX-M encoding plasmids present during the early phase of the ESBL pandemic in western Sweden.Scientific reports · 2024Article
- Strain-level bacterial typing directly from patient samples using optical DNA mapping.Communications medicine · 2023Article
- Identification and characterization of plasmids carrying the mobile colistin resistance geneJAC-antimicrobial resistance · 2023Article
- A simple cut and stretch assay to detect antimicrobial resistance genes on bacterial plasmids by single-molecule fluorescence microscopy.Scientific reports · 2022Article
- Article
- Optical DNA Mapping of Plasmids Reveals Clonal Spread of Carbapenem-ResistantAntibiotics (Basel, Switzerland) · 2021Article
- Identity ofMicroorganisms · 2021Article
- Single-molecule optical genome mapping in nanochannels: multidisciplinarity at the nanoscale.Essays in biochemistry · 2021Article
- Detection of structural variations in densely-labelled optical DNA barcodes: A hidden Markov model approach.PloS one · 2021Article
- Combining dense and sparse labeling in optical DNA mapping.PloS one · 2021Article
- Article
- Real-time compaction of nanoconfined DNA by an intrinsically disordered macromolecular counterion.Biochemical and biophysical research communications · 2020Article
- Optical maps of plasmids as a proxy for clonal spread of MDR bacteria: a case study of an outbreak in a rural Ethiopian hospital.The Journal of antimicrobial chemotherapy · 2020Article
- Phosphorylated CtIP bridges DNA to promote annealing of broken ends.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Cultivation-Free Typing of Bacteria Using Optical DNA Mapping.ACS infectious diseases · 2020Article
- Identifying microbial species by single-molecule DNA optical mapping and resampling statistics.NAR genomics and bioinformatics · 2020Article
- Truncated TALE-FP as DNA Staining Dye in a High-salt Buffer.Scientific reports · 2019Article
- Enzyme-free optical DNA mapping of the human genome using competitive binding.Nucleic acids research · 2019Article
- Article
Corrections and comments
- Erratum issued
Authors and funding
15 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The rapid spread of antibiotic resistance - currently one of the greatest threats to human health according to WHO - is to a large extent enabled by plasmid-mediated horizontal transfer of resistance genes. Rapid identification and characterization of plasmids is thus important both for individual clinical outcomes and for epidemiological monitoring of antibiotic resistance. Toward this aim, we have developed an optical DNA mapping procedure where individual intact plasmids are elongated within nanofluidic channels and visualized through fluorescence microscopy, yielding barcodes that reflect the underlying sequence. The assay rapidly identifies plasmids through statistical comparisons with barcodes based on publicly available sequence repositories and also enables detection of structural variations. Since the assay yields holistic sequence information for individual intact plasmids, it is an ideal complement to next generation sequencing efforts which involve reassembly of sequence reads from fragmented DNA molecules. The assay should be applicable in microbiology labs around the world in applications ranging from fundamental plasmid biology to clinical epidemiology and diagnostics.
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