Detection and Characterization of Rare Circulating Endothelial Cells by Imaging Flow Cytometry.

Leigh Samsel, J Philip McCoy

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it

0cells of the map it votes in

2citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

Analysis of the Leishmania mexicana promastigote cell cycle using imaging flow cytometry provides new insights into cell cycle flexibility and events of short duration.PloS one · 2024
Article
Gold Nanoparticles in Single-Cell Analysis for Surface Enhanced Raman Scattering.Molecules (Basel, Switzerland) · 2016
Review

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Leigh SamselNHLBI Flow Cytometry Core Facility, NHLBI, NIH, Building 31, Room 5A52, MSC 2486, 31 Center Drive, Bethesda, MD, 20892, USA.

J Philip McCoyNHLBI Flow Cytometry Core Facility, NHLBI, NIH, Building 31, Room 5A52, MSC 2486, 31 Center Drive, Bethesda, MD, 20892, USA. mccoyj@nhlbi.nih.gov.

Funding

Flow Cytometry Core FacilityZICHL005905 · NHLBI · NATIONAL HEART, LUNG, AND BLOOD INSTITUTE · PI DAGUR, PRADEEP · 2009 to 2025

$32.3M

Intramural NIH HHS ZIC HL005905

6 · The paper itself

Abstract

Circulating endothelial cells (CECs) are angiogenic cells that appear in increased numbers in the peripheral circulation either as a result of vascular injury or in response to angiogenic stimuli. Elevated levels of CECs have been correlated with various disease states, indicating the use of CECs as a biomarker of disease. Flow cytometry is a widely accepted method for detecting and quantitating CECs. Flow cytometry provides statistical information on large numbers of cells but no information on morphological characteristics. Imaging flow cytometry combines traditional flow cytometry and microscopy, providing a streamlined, multiparameter approach to characterize the biological properties and morphology of large numbers of cells, and is particularly amenable for rare event analysis such as CECs. This approach for identifying and characterizing CECs allows the morphological characterization of large numbers of live, nucleated, single CECs, and alleviates the need for prior enrichment.

Indexed as

Endothelial CellsFlow CytometryHumansImage CytometryCD146CECsCirculating endothelial cellsImaging flow cytometryRare event flow cytometry

Identifiers

PMID27460251

DOI10.1007/978-1-4939-3302-0_18

PMCPMC6471516

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.