ArticlePloS one2016
Direct and Osmolarity-Dependent Effects of Glycine on Preimplantation Bovine Embryos.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 30 citations in OpenAlex.
- Glycine as a Metabolic Regulator of Reproductive Function in Livestock: From Gametes to Early Embryos.Animals : an open access journal from MDPI · 2026Review
- Bridging the gap between basic research and commercial application at scale for in vitro bovine embryo production.Animal reproduction · 2026Article
- In vitro production significantly reduces metabolic differences among bovine embryos.Metabolomics : Official journal of the Metabolomic Society · 2025Article
- The composition of commercially available human embryo culture media.Human reproduction (Oxford, England) · 2025Article
- Stage-dependent changes in culture medium osmolality promote porcine oocyte maturationFrontiers in cell and developmental biology · 2025Article
- In vitro culture alters cell lineage composition and cellular metabolism of bovine blastocyst†.Biology of reproduction · 2024Article
- Osmolar Modulation Drives Reversible Cell Cycle Exit and Human Pluripotent Cell Differentiation via NF-κВ and WNT Signaling.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Bovine embryo productionAnimal reproduction · 2024Review
- Lipid Enriched Reduced Nutrient Culture Medium Improves Bovine Blastocyst Formation.Reproduction & fertility · 2023Article
- Glutaminolysis is involved in the activation of mTORC1 in in vitro-produced porcine embryos.Molecular reproduction and development · 2021Article
- Vitamin and mineral supplementation and rate of gain during the first trimester of gestation affect concentrations of amino acids in maternal serum and allantoic fluid of beef heifers.Journal of animal science · 2021Article
- Non-Invasive Human Embryo Metabolic Assessment as a Developmental Criterion.Journal of clinical medicine · 2020Article
- Effect of evaporation-induced osmotic changes in culture media in a dry-type incubator on clinical outcomes in in vitro fertilization-embryo transfer cycles.Clinical and experimental reproductive medicine · 2020Article
- Developmental and molecular response of bovine embryos to reduced nutrientsReproduction & fertility · 2020Article
- A novel culture medium with reduced nutrient concentrations supports the development and viability of mouse embryos.Scientific reports · 2020Article
- Role of bone morphogenetic protein signaling in bovine early embryonic development and stage specific embryotropic actions of follistatin†.Biology of reproduction · 2020Article
- Spatial and Pregnancy-Related Changes in the Protein, Amino Acid, and Carbohydrate Composition of Bovine Oviduct Fluid.International journal of molecular sciences · 2020Article
- Metabolic Profiling in Blastocoel Fluid and Blood Plasma of Diabetic Rabbits.International journal of molecular sciences · 2020Article
- Metabolomic Profile of Oviductal Extracellular Vesicles across the Estrous Cycle in Cattle.International journal of molecular sciences · 2019Article
- Interferon tau-dependent and independent effects of the bovine conceptus on the endometrial transcriptome†.Biology of reproduction · 2019Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Concentrations of glycine (Gly) in embryo culture media are often lower (~0.1 mM) than those in oviductal or uterine fluids (≥1.2 mM). The objective of this study was to determine direct and osmolarity-dependent effects of physiological concentrations of Gly on blastocyst formation and hatching, cell allocation to the trophectoderm (TE) and inner cell mass (ICM), and metabolic activity of bovine embryos. In experiment 1, zygotes were cultured with 100 or 120 mM NaCl and 0 or 1 mM Gly for the first 72 h of culture. Blastocyst formation and hatching were improved (P<0.05) when embryos were cultured with 100 compared to 120 mM NaCl. Inclusion of 1 mM Gly improved (P<0.05) blastocyst formation compared to 0 mM Gly, but this effect was only significant (P<0.05) for embryos cultured with 120 mM NaCl, suggesting bovine embryos can utilize Gly as an osmolyte. In experiment 2, embryos were cultured with 0.1, 1.1, 2.1, or 4.1 mM Gly (100 mM NaCl) for the final 96 h of culture. Blastocyst development was not affected (P>0.05) by Gly, but hatching (0.1 mM Gly, 18.2%) was improved (P<0.05) when embryos were cultured with 1.1 (31.4%) or 2.1 (29.4%) mM Gly. Blastocyst, TE, and ICM cell numbers were not affected (P>0.05) by Gly in either experiment. Blastocysts produced alanine, glutamine, pyruvate, and urea and consumed aspartate, but this metabolic profile was not affected (P>0.05) by Gly. In conclusion, Gly (1.0 mM) improves the development of both early and late stage embryos, but beneficial effects are more pronounced for early embryos exposed to elevated osmolarity.
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