Evidence map›Paper›PMID 27459314›Full record

ArticleEndocrinology2016

Overexpression of Lnk in the Ovaries Is Involved in Insulin Resistance in Women With Polycystic Ovary Syndrome.

Meihua Hao, Feng Yuan, Chenchen Jin, Zehong Zhou, Qi Cao, Ling Xu, Guanlei Wang, Hui Huang, Dongzi Yang, Meiqing Xie and 1 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Endocrinology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
1.3field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 19 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 5 institutions in 2 countries.

Meihua HaoDepartment of Obstetrics and Gynecology (M.H., H.H., D.Y., M.X., X.Z.), Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 510120 China; Department of Pharmacology (F.Y., C.J., G.W.), Cardiac and Cerebral Vascular Research Center, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, 510120 China; Department of Obstetrics and Gynecology (Z.Z.), Reproductive Medical Center, Peking University Third Hospital, Haidian District, Beijing, China; Division of Hematology/Oncology (Q.C.), Cedar-Sinai Medical Center, University of California, Los Angeles School of Medicine, Los Angeles, California; and Cedar-Sinai Medical Center (L.X.), University of California, Los Angeles School of Medicine, Los Angeles, California.
Feng YuanDepartment of Obstetrics and Gynecology (M.H., H.H., D.Y., M.X., X.Z.), Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 510120 China; Department of Pharmacology (F.Y., C.J., G.W.), Cardiac and Cerebral Vascular Research Center, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, 510120 China; Department of Obstetrics and Gynecology (Z.Z.), Reproductive Medical Center, Peking University Third Hospital, Haidian District, Beijing, China; Division of Hematology/Oncology (Q.C.), Cedar-Sinai Medical Center, University of California, Los Angeles School of Medicine, Los Angeles, California; and Cedar-Sinai Medical Center (L.X.), University of California, Los Angeles School of Medicine, Los Angeles, California.
Chenchen JinDepartment of Obstetrics and Gynecology (M.H., H.H., D.Y., M.X., X.Z.), Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 510120 China; Department of Pharmacology (F.Y., C.J., G.W.), Cardiac and Cerebral Vascular Research Center, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, 510120 China; Department of Obstetrics and Gynecology (Z.Z.), Reproductive Medical Center, Peking University Third Hospital, Haidian District, Beijing, China; Division of Hematology/Oncology (Q.C.), Cedar-Sinai Medical Center, University of California, Los Angeles School of Medicine, Los Angeles, California; and Cedar-Sinai Medical Center (L.X.), University of California, Los Angeles School of Medicine, Los Angeles, California.
Zehong ZhouDepartment of Obstetrics and Gynecology (M.H., H.H., D.Y., M.X., X.Z.), Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 510120 China; Department of Pharmacology (F.Y., C.J., G.W.), Cardiac and Cerebral Vascular Research Center, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, 510120 China; Department of Obstetrics and Gynecology (Z.Z.), Reproductive Medical Center, Peking University Third Hospital, Haidian District, Beijing, China; Division of Hematology/Oncology (Q.C.), Cedar-Sinai Medical Center, University of California, Los Angeles School of Medicine, Los Angeles, California; and Cedar-Sinai Medical Center (L.X.), University of California, Los Angeles School of Medicine, Los Angeles, California.
Qi CaoDepartment of Obstetrics and Gynecology (M.H., H.H., D.Y., M.X., X.Z.), Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 510120 China; Department of Pharmacology (F.Y., C.J., G.W.), Cardiac and Cerebral Vascular Research Center, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, 510120 China; Department of Obstetrics and Gynecology (Z.Z.), Reproductive Medical Center, Peking University Third Hospital, Haidian District, Beijing, China; Division of Hematology/Oncology (Q.C.), Cedar-Sinai Medical Center, University of California, Los Angeles School of Medicine, Los Angeles, California; and Cedar-Sinai Medical Center (L.X.), University of California, Los Angeles School of Medicine, Los Angeles, California.
Ling XuDepartment of Obstetrics and Gynecology (M.H., H.H., D.Y., M.X., X.Z.), Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 510120 China; Department of Pharmacology (F.Y., C.J., G.W.), Cardiac and Cerebral Vascular Research Center, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, 510120 China; Department of Obstetrics and Gynecology (Z.Z.), Reproductive Medical Center, Peking University Third Hospital, Haidian District, Beijing, China; Division of Hematology/Oncology (Q.C.), Cedar-Sinai Medical Center, University of California, Los Angeles School of Medicine, Los Angeles, California; and Cedar-Sinai Medical Center (L.X.), University of California, Los Angeles School of Medicine, Los Angeles, California.
Guanlei WangDepartment of Obstetrics and Gynecology (M.H., H.H., D.Y., M.X., X.Z.), Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 510120 China; Department of Pharmacology (F.Y., C.J., G.W.), Cardiac and Cerebral Vascular Research Center, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, 510120 China; Department of Obstetrics and Gynecology (Z.Z.), Reproductive Medical Center, Peking University Third Hospital, Haidian District, Beijing, China; Division of Hematology/Oncology (Q.C.), Cedar-Sinai Medical Center, University of California, Los Angeles School of Medicine, Los Angeles, California; and Cedar-Sinai Medical Center (L.X.), University of California, Los Angeles School of Medicine, Los Angeles, California.
Hui HuangDepartment of Obstetrics and Gynecology (M.H., H.H., D.Y., M.X., X.Z.), Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 510120 China; Department of Pharmacology (F.Y., C.J., G.W.), Cardiac and Cerebral Vascular Research Center, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, 510120 China; Department of Obstetrics and Gynecology (Z.Z.), Reproductive Medical Center, Peking University Third Hospital, Haidian District, Beijing, China; Division of Hematology/Oncology (Q.C.), Cedar-Sinai Medical Center, University of California, Los Angeles School of Medicine, Los Angeles, California; and Cedar-Sinai Medical Center (L.X.), University of California, Los Angeles School of Medicine, Los Angeles, California.
Dongzi YangDepartment of Obstetrics and Gynecology (M.H., H.H., D.Y., M.X., X.Z.), Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 510120 China; Department of Pharmacology (F.Y., C.J., G.W.), Cardiac and Cerebral Vascular Research Center, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, 510120 China; Department of Obstetrics and Gynecology (Z.Z.), Reproductive Medical Center, Peking University Third Hospital, Haidian District, Beijing, China; Division of Hematology/Oncology (Q.C.), Cedar-Sinai Medical Center, University of California, Los Angeles School of Medicine, Los Angeles, California; and Cedar-Sinai Medical Center (L.X.), University of California, Los Angeles School of Medicine, Los Angeles, California.
Meiqing XieDepartment of Obstetrics and Gynecology (M.H., H.H., D.Y., M.X., X.Z.), Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 510120 China; Department of Pharmacology (F.Y., C.J., G.W.), Cardiac and Cerebral Vascular Research Center, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, 510120 China; Department of Obstetrics and Gynecology (Z.Z.), Reproductive Medical Center, Peking University Third Hospital, Haidian District, Beijing, China; Division of Hematology/Oncology (Q.C.), Cedar-Sinai Medical Center, University of California, Los Angeles School of Medicine, Los Angeles, California; and Cedar-Sinai Medical Center (L.X.), University of California, Los Angeles School of Medicine, Los Angeles, California.
Xiaomiao ZhaoDepartment of Obstetrics and Gynecology (M.H., H.H., D.Y., M.X., X.Z.), Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University, Guangzhou, 510120 China; Department of Pharmacology (F.Y., C.J., G.W.), Cardiac and Cerebral Vascular Research Center, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou, 510120 China; Department of Obstetrics and Gynecology (Z.Z.), Reproductive Medical Center, Peking University Third Hospital, Haidian District, Beijing, China; Division of Hematology/Oncology (Q.C.), Cedar-Sinai Medical Center, University of California, Los Angeles School of Medicine, Los Angeles, California; and Cedar-Sinai Medical Center (L.X.), University of California, Los Angeles School of Medicine, Los Angeles, California.
Sun Yat-sen University · CNSun Yat-sen Memorial Hospital · CNCedars-Sinai Medical Center · USPeking University · CNUniversity of California, Los Angeles · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Polycystic ovary syndrome (PCOS) progression involves abnormal insulin signaling. SH2 domain-containing adaptor protein (Lnk) may be an important regulator of the insulin signaling pathway. We investigated whether Lnk was involved in insulin resistance (IR). Thirty-seven women due to receive laparoscopic surgery from June 2011 to February 2012 were included from the gynecologic department of the Sun Yat-Sen Memorial Hospital, Sun Yat-Sen University. Samples of polycystic and normal ovary tissues were examined by immunohistochemistry. Ovarian cell lines underwent insulin stimulation and Lnk overexpression. Expressed Lnk underwent coimmunoprecipitation tests with green fluorescent protein-labeled insulin receptor and His-tagged insulin receptor substrate 1 (IRS1), and their colocalization in HEK293T cells was examined. Ovarian tissues from PCOS patients with IR exhibited higher expression of Lnk than ovaries from normal control subjects and PCOS patients without IR; mainly in follicular granulosa cells, the follicular fluid and plasma of oocytes in secondary follicles, and atretic follicles. Lnk was coimmunoprecipitated with insulin receptor and IRS1. Lnk and insulin receptor/IRS1 locations overlapped around the nucleus. IR, protein kinase B (Akt), and ERK1/2 activities were inhibited by Lnk overexpression and inhibited further after insulin stimulation, whereas IRS1 serine activity was increased. Insulin receptor (Tyr1150/1151), Akt (Thr308), and ERK1/2 (Thr202/Tyr204) phosphorylation was decreased, whereas IRS1 (Ser307) phosphorylation was increased with Lnk overexpression. In conclusion, Lnk inhibits the phosphatidylinositol 3 kinase-AKT and MAPK-ERK signaling response to insulin. Higher expression of Lnk in PCOS suggests that Lnk probably plays a role in the development of IR.

Indexed as

Insulin ResistanceAdaptor Proteins, Signal TransducingAdultCase-Control StudiesCell Line, TumorFemaleHumansInsulin Receptor Substrate ProteinsIntracellular Signaling Peptides and ProteinsMAP Kinase Signaling SystemOvaryPhosphatidylinositol 3-KinasesPolycystic Ovary SyndromeProteinsProto-Oncogene Proteins c-aktReceptor, InsulinAdaptor Proteins, Signal TransducingInsulin Receptor Substrate ProteinsIntracellular Signaling Peptides and ProteinsIRS1 protein, humanPhosphatidylinositol 3-KinasesProteinsProto-Oncogene Proteins c-aktReceptor, InsulinSH2B3 protein, human

Identifiers

PMID27459314
PMCPMC5045500
OpenAlexW2488959709

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.