ArticlePloS one2016
Structure of Alcohol Oxidase from Pichia pastoris by Cryo-Electron Microscopy.
Article in PloS one, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 47 citations in OpenAlex.
- Evolution of alcohol oxidase for improved methanol bioconversion and formaldehyde tolerance.Synthetic and systems biotechnology · 2027Article
- Visualisation of peroxisomes: a journey through seven decades.Histochemistry and cell biology · 2026Review
- Review
- Cryo-EM Structures of Alcohol Oxidase Isozymes Reveal Structural Determinants of Cofactor Variation and Enzymatic Activity in Ogataea methanolica.Microbial biotechnology · 2026Article
- Sequence and taxonomic feature evaluation facilitated the discovery of alcohol oxidases.Synthetic and systems biotechnology · 2025Article
- Peroxisomal core structures segregate diverse metabolic pathways.Nature communications · 2025Article
- Engineering the methylotrophic yeastFrontiers in bioengineering and biotechnology · 2023Article
- Properties, Physiological Functions and Involvement of Basidiomycetous Alcohol Oxidase in Wood Degradation.International journal of molecular sciences · 2022Review
- Kinetically restrained oxygen reduction to hydrogen peroxide with nearly 100% selectivity.Nature communications · 2022Article
- Three-dimensional structure determination of protein complexes using matrix-landing mass spectrometry.Nature communications · 2022Article
- Transcutaneous Flexible Sensor forACS sensors · 2022Article
- What makes Komagataella phaffii non-conventional?FEMS yeast research · 2021Review
- Article
- Localization of ligands within human carbonic anhydrase II usingChemical science · 2019Article
- Structure-Based Engineering of Phanerochaete chrysosporium Alcohol Oxidase for Enhanced Oxidative Power toward Glycerol.Biochemistry · 2018Article
- Multiplicity of enzymatic functions in the CAZy AA3 family.Applied microbiology and biotechnology · 2018Review
- Enzymatic Preparation of 2,5-Furandicarboxylic Acid (FDCA)-A Substitute of Terephthalic Acid-By the Joined Action of Three Fungal Enzymes.Microorganisms · 2018Article
- Cryo-FIB specimen preparation for use in a cartridge-type cryo-TEM.Journal of structural biology · 2017Article
- Trends in the Electron Microscopy Data Bank (EMDB).Acta crystallographica. Section D, Structural biology · 2017Article
- Effect of cell retention techniques in Komagataella phaffii lab-scale continuous processes.Biotechnology progressArticle
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The first step in methanol metabolism in methylotrophic yeasts, the oxidation of methanol and higher alcohols with molecular oxygen to formaldehyde and hydrogen peroxide, is catalysed by alcohol oxidase (AOX), a 600-kDa homo-octamer containing eight FAD cofactors. When these yeasts are grown with methanol as the carbon source, AOX forms large crystalline arrays in peroxisomes. We determined the structure of AOX by cryo-electron microscopy at a resolution of 3.4 Å. All residues of the 662-amino acid polypeptide as well as the FAD are well resolved. AOX shows high structural homology to other members of the GMC family of oxidoreductases, which share a conserved FAD binding domain, but have different substrate specificities. The preference of AOX for small alcohols is explained by the presence of conserved bulky aromatic residues near the active site. Compared to the other GMC enzymes, AOX contains a large number of amino acid inserts, the longest being 75 residues. These segments are found at the periphery of the monomer and make extensive inter-subunit contacts which are responsible for the very stable octamer. A short surface helix forms contacts between two octamers, explaining the tendency of AOX to form crystals in the peroxisomes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.