ArticleGastroenterology and hepatology from bed to bench2016
Creation of a murine orthotopic hepatoma model with intra-abdominal metastasis.
Article in Gastroenterology and hepatology from bed to bench, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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1 citing paper in PubMed.
- Targeted multicolor in vivo imaging over 1,000 nm enabled by nonamethine cyanines.Nature methods · 2022Article
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Authors and funding
3 authors.
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Abstract
aimTo create an orthotopic hepatoma model with local metastasis monitored with ultrasound could be created as a platform for testing new treatments.
backgroundHepatoma accounts for 25% of liver tumors in children with poor overall survival. Intraabdominal metastasis are present in 35% of patients at time of diagnosis. We hypothesized that an orthotopic tumor model with local metastasis could be created as a platform for testing treatment modalities and could be monitored with ultrasound. PATIENTS AND
methodsOne million human hepatoma cells (Hep3B) were injected into the left lobe of the liver of immunocompromised mice. Tumor volume was monitored with high frequency-ultrasound until it reached 1,000mm(3). At that time animals were sacrificed and examined for gross metastatic disease. Tumor sections were analyzed with hematoxylin and eosin (H&E) staining.
resultsTumor formed in 8/15 mice. The tumor was detected as small as 19.59mm(3) on ultrasound. Of the forming tumors, tumor size was 145±177.93mm(3) at 60 days post-injection, 665±650.39mm(3) at 67 days, and reached >1000mm(3) by 76.6±9.9 days. At necropsy, four mice (50%) had tumor only within the liver, four (50%) had additional tumors in omentum, pelvis and peritoneum. H&E showed tumor within the normal liver parenchyma, with multiple mitotic figures, small areas of necrosis, and hemorrhage within the tumor.
conclusionWe have successfully established an orthotopic hepatoma murine model, with a local metastatic rate of 50%. Non-invasive tumor monitoring is feasible via ultrasound.
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