ArticleFrontiers in microbiology2016
Industrial Acetogenic Biocatalysts: A Comparative Metabolic and Genomic Analysis.
Article in Frontiers in microbiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 108 citations in OpenAlex.
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- Overexpression of endogenous aldehyde:ferredoxin oxidoreductase genes enhances ethanol biosynthesis in Clostridium autoethanogenum.World journal of microbiology & biotechnology · 2026Article
- The potential of syngas as a central mediator for a carbon-agnostic circular economy.Nature communications · 2026Review
- Enhancing microbial electrosynthesis of fatty acids from carbon dioxide by combining bioaugmentation of an acetogenic enrichment and Fe-Sn oxide cathode coating.RSC advances · 2026Article
- Careful CO Addition Enhances Autotrophic d-Lactate Formation With EngineeredEngineering in life sciences · 2026Article
- Genomic insights into Thermomonas hydrothermalis: potential applications in industrial biotechnology.World journal of microbiology & biotechnology · 2025Article
- Recent progress in engineeringSynthetic and systems biotechnology · 2024Review
- Probing efficient microbial COMicrobial biotechnology · 2024Article
- Engineered acetogenic bacteria as microbial cell factory for diversified biochemicals.Frontiers in bioengineering and biotechnology · 2024Review
- Translational efficiency in gas-fermenting bacteria: Adding a new layer of regulation to gene expression in acetogens.iScience · 2023Review
- Design and validation of a multiplex PCR method for the simultaneous quantification of Clostridium acetobutylicum, Clostridium carboxidivorans and Clostridium cellulovorans.Scientific reports · 2023Article
- The potential of CONature communications · 2023Review
- Metabolic engineering of Clostridium autoethanogenum for ethyl acetate production from CO.Microbial cell factories · 2022Article
- Genome-Scale Mining of Acetogens of the GenusMicrobiology spectrum · 2022Article
- Metabolic engineering of Clostridium ljungdahlii for the production of hexanol and butanol from COMicrobial cell factories · 2022Article
- Metabolic Engineering Interventions for Sustainable 2,3-Butanediol Production in Gas-FermentingmSystems · 2022Article
- Comparison of Syngas-FermentingMicroorganisms · 2022Article
- Continuous sulfide supply enhanced autotrophic production of alcohols with Clostridium ragsdalei.Bioresources and bioprocessing · 2022Article
- Agr Quorum Sensing influences the Wood-Ljungdahl pathway in Clostridium autoethanogenum.Scientific reports · 2022Article
- Engineering Acetogenic Bacteria for Efficient One-Carbon Utilization.Frontiers in microbiology · 2022Review
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Synthesis gas (syngas) fermentation by anaerobic acetogenic bacteria employing the Wood-Ljungdahl pathway is a bioprocess for production of biofuels and biocommodities. The major fermentation products of the most relevant biocatalytic strains (Clostridium ljungdahlii, C. autoethanogenum, C. ragsdalei, and C. coskatii) are acetic acid and ethanol. A comparative metabolic and genomic analysis using the mentioned biocatalysts might offer targets for metabolic engineering and thus improve the production of compounds apart from ethanol. Autotrophic growth and product formation of the four wild type (WT) strains were compared in uncontrolled batch experiments. The genomes of C. ragsdalei and C. coskatii were sequenced and the genome sequences of all four biocatalytic strains analyzed in comparative manner. Growth and product spectra (acetate, ethanol, 2,3-butanediol) of C. autoethanogenum, C. ljungdahlii, and C. ragsdalei were rather similar. In contrast, C. coskatii produced significantly less ethanol and its genome sequence lacks two genes encoding aldehyde:ferredoxin oxidoreductases (AOR). Comparative genome sequence analysis of the four WT strains revealed high average nucleotide identity (ANI) of C. ljungdahlii and C. autoethanogenum (99.3%) and C. coskatii (98.3%). In contrast, C. ljungdahlii WT and C. ragsdalei WT showed an ANI-based similarity of only 95.8%. Additionally, recombinant C. ljungdahlii strains were constructed that harbor an artificial acetone synthesis operon (ASO) consisting of the following genes: adc, ctfA, ctfB, and thlA (encoding acetoacetate decarboxylase, acetoacetyl-CoA:acetate/butyrate:CoA-transferase subunits A and B, and thiolase) under the control of thlA promoter (P thlA ) from C. acetobutylicum or native pta-ack promoter (P pta-ack ) from C. ljungdahlii. Respective recombinant strains produced 2-propanol rather than acetone, due to the presence of a NADPH-dependent primary-secondary alcohol dehydrogenase that converts acetone to 2-propanol. Furthermore, the ClosTron(TM) system was used to construct an adhE1 integration mutant. These results provide extensive insights into genetic features of industrially relevant bacterial biocatalysts and expand the toolbox for metabolic engineering of acetogenic bacteria able to ferment syngas.
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