ArticleFrontiers in neuroscience2016
A Simplified In vitro Experimental Model Encompasses the Essential Features of Sleep.
Article in Frontiers in neuroscience, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 34 citations in OpenAlex.
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- Review
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- Learning populations with hubs govern the initiation and propagation of spontaneous bursts in neuronal networks after learning.Frontiers in neuroscience · 2022Article
- The role of calcium and CaMKII in sleep.Frontiers in systems neuroscience · 2022Review
- Spontaneous and Perturbational Complexity in Cortical Cultures.Brain sciences · 2021Article
- meaRtools: An R package for the analysis of neuronal networks recorded on microelectrode arrays.PLoS computational biology · 2018Article
- In vitro Cortical Network Firing is Homeostatically Regulated: A Model for Sleep Regulation.Scientific reports · 2018Article
- Activity changes in neuron-astrocyte networks in culture under the effect of norepinephrine.PloS one · 2018Article
- Theta rhythm-like bidirectional cycling dynamics of living neuronal networks in vitro.PloS one · 2018Article
- Stimulation triggers endogenous activity patterns in cultured cortical networks.Scientific reports · 2017Article
- Article
Corrections and comments
- Erratum issued
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this paper, we show that neuronal assemblies plated on Micro Electrode Arrays present synchronized, low frequency firing patterns similar to in vivo slow wave oscillations, which are a key parameter of sleep-like state. Although neuronal cultures lack the characteristic high-frequency waves of wakefulness, it is possible to modulate their spontaneous firing pattern through the administration of specific neurotransmitters such as acetylcholine. We thus stimulated the cortical cultures with an agonist of acetylcholine receptor, Carbachol, which caused a desynchronization of the spontaneous firing of the cultures. We recorded and monitored the cultures for a period of over 31 h. We analyzed the electrophysiological signals by exploiting novel methodological approaches, taking into account the different temporal scales of the recorded signals, and considering both spikes and local field potentials. Supporting the electrophysiological analysis results, gene expressions of targeted genes showed the activation of specific markers involved in sleep-wake rhythms. Our results demonstrate that the Carbachol treatment induces desynchronization of neuronal activity, altering sleep-like properties in an in vitro model.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.