ArticleFree radical biology & medicine2016
On the use of peroxy-caged luciferin (PCL-1) probe for bioluminescent detection of inflammatory oxidants in vitro and in vivo - Identification of reaction intermediates and oxidant-specific minor products.
Article in Free radical biology & medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 49 citations in OpenAlex.
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- Phenyl Radical-Mediated Fluorogenic Cyclization for Specific Detection of Peroxynitrite.Analytical chemistry · 2025Article
- Small molecule probes for peroxynitrite detection.Redox biochemistry and chemistry · 2024Article
- Boronate-Based Bioactive Compounds Activated by Peroxynitrite and Hydrogen Peroxide.Redox biochemistry and chemistry · 2024Article
- The oxygen puzzle in FLASH radiotherapy: A comprehensive review and experimental outlook.Clinical and translational radiation oncology · 2024Review
- Boronate-Based Oxidant-Responsive Derivatives of Acetaminophen as Proinhibitors of Myeloperoxidase.Chemical research in toxicology · 2023Article
- Review of optical reporters of radiation effectsJournal of biomedical optics · 2023Review
- Polyphenolic Boronates Inhibit Tumor Cell Proliferation: Potential Mitigators of Oxidants in the Tumor Microenvironment.Cancers · 2023Article
- Fluorescent probes for monitoring myeloperoxidase-derived hypochlorous acid: a comparative study.Scientific reports · 2022Article
- Chemiluminescence Measurement of Reactive Sulfur and Nitrogen Species.Antioxidants & redox signaling · 2022Review
- The Chemistry of HNO: Mechanisms and Reaction Kinetics.Frontiers in chemistry · 2022Review
- Kinetics of Azanone (HNO) Reactions with Thiols: Effect of pH.Cell biochemistry and biophysics · 2021Article
- Novel Boronate Probe Based on 3-Benzothiazol-2-yl-7-hydroxy-chromen-2-one for the Detection of Peroxynitrite and Hypochlorite.Molecules (Basel, Switzerland) · 2021Article
- Advances in Imaging Reactive Oxygen Species.Journal of nuclear medicine : official publication, Society of Nuclear Medicine · 2021Review
- Tracking isotopically labeled oxidants using boronate-based redox probes.The Journal of biological chemistry · 2020Article
- Increased formation of reactive oxygen species during tumor growth: Ex vivo low-temperature EPR and in vivo bioluminescence analyses.Free radical biology & medicine · 2020Article
- Boronate-Based Probes for Biological Oxidants: A Novel Class of Molecular Tools for Redox Biology.Frontiers in chemistry · 2020Review
- Small-molecule luminescent probes for the detection of cellular oxidizing and nitrating species.Free radical biology & medicine · 2018Review
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Authors and funding
5 authors at 3 institutions in 3 countries.
Funding
Abstract
Peroxy-caged luciferin (PCL-1) probe was first used to image hydrogen peroxide in living systems (Van de Bittner et al., 2010 [9]). Recently this probe was shown to react with peroxynitrite more potently than with hydrogen peroxide (Sieracki et al., 2013 [11]) and was suggested to be a more suitable probe for detecting peroxynitrite under in vivo conditions. In this work, we investigated in detail the products formed from the reaction between PCL-1 and hydrogen peroxide, hypochlorite, and peroxynitrite. HPLC analysis showed that hydrogen peroxide reacts slowly with PCL-1, forming luciferin as the only product. Hypochlorite reaction with PCL-1 yielded significantly less luciferin, as hypochlorite oxidized luciferin to form a chlorinated luciferin. Reaction between PCL-1 and peroxynitrite consists of a major and minor pathway. The major pathway results in luciferin and the minor pathway produces a radical-mediated nitrated luciferin. Radical intermediate was characterized by spin trapping. We conclude that monitoring of chlorinated and nitrated products in addition to bioluminescence in vivo will help identify the nature of oxidant responsible for bioluminescence derived from PCL-1.
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Registered trials
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