ArticleProceedings of the National Academy of Sciences of the United States of America2016
Complete in vitro generation of fertile oocytes from mouse primordial germ cells.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 102 papers.
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Who cites it
102 citing papers in PubMed, 190 citations in OpenAlex.
- Characterization of the metabolome and proteome of stem cell-derived human primordial germ cell-like cells: a multi-omics approach.Biology open · 2026Article
- Article
- Ovarian and follicular culture enables the production of fertile oocytes in C57BL/6 mice.The Journal of reproduction and development · 2026Article
- Ovarian aging: maintenance and activation of the primordial follicle pool.Journal of ovarian research · 2026Review
- Stem Cells and Wildlife Conservation.Advances in experimental medicine and biology · 2026Review
- Trans-generational maintenance of mitochondrial DNA integrity in oocytes during early folliculogenesis.PLoS genetics · 2025Article
- In vitro system completely restores oogenesis in congenitally infertile mice.Human reproduction (Oxford, England) · 2025Article
- In Vitro Gamete Production in Mammals: Decades of Pioneering Approaches in Germ Cell and Stem Cell Biology Supporting Innovative Discovery and Expansion of Human Reproductive Potential.Molecular reproduction and development · 2025Review
- Optimization of insulin-like growth factor-1 supplementation enhances oocyte quality by modulating oxidative stress and apoptotic pathways duringVeterinary world · 2025Article
- A visualized and quercetin-optimized three-dimensional culture model of mouse ovaries derived from fetal gonads.Acta biochimica et biophysica Sinica · 2025Article
- Article
- Optimization of ovum pick-up-in vitro fertilization and in vitro growth of immature oocytes in ruminants.The Journal of reproduction and development · 2025Review
- In vitro gametogenesis: Towards competent oocytes: Limitations and future improvements for generating oocytes from pluripotent stem cells in culture.BioEssays : news and reviews in molecular, cellular and developmental biology · 2025Review
- Review
- A tug-of-war between germ cell motility and intercellular bridges controls germline cyst formation in mice.Current biology : CB · 2024Article
- Luteinizing Hormone Receptor Mutation (LHRInterdisciplinary sciences, computational life sciences · 2024Article
- In vitro gametogenesis (IVG): reflections from a workshop.Journal of assisted reproduction and genetics · 2024Article
- In vitro production of viable eggs from undeveloped oocytes in mouse preantral follicles by reconstructing granulosa cell-oocyte complexes†.Biology of reproduction · 2024Article
- Secondary follicles enable efficient germline mtDNA base editing at hard-to-edit site.Molecular therapy. Nucleic acids · 2024Article
- In Vitro Growth of Mammalian Follicles and Oocytes.Animals : an open access journal from MDPI · 2024Article
42 more citing papers are in PubMed but not listed here.
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Reconstituting gametogenesis in vitro is a key goal for reproductive biology and regenerative medicine. Successful in vitro reconstitution of primordial germ cells and spermatogenesis has recently had a significant effect in the field. However, recapitulation of oogenesis in vitro remains unachieved. Here we demonstrate the first reconstitution, to our knowledge, of the entire process of mammalian oogenesis in vitro from primordial germ cells, using an estrogen-receptor antagonist that promotes normal follicle formation, which in turn is crucial for supporting oocyte growth. The fundamental events in oogenesis (i.e., meiosis, oocyte growth, and genomic imprinting) were reproduced in the culture system. The most rigorous evidence of the recapitulation of oogenesis was the birth of fertile offspring, with a maximum of seven pups obtained from a cultured gonad. Moreover, cryopreserved gonads yielded functional oocytes and offspring in this culture system. Thus, our in vitro system will enable both innovative approaches for a deeper understanding of oogenesis and a new avenue to create and preserve female germ cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.