ArticleScientific reports2016
Reliability of DNA methylation measures from dried blood spots and mononuclear cells using the HumanMethylation450k BeadArray.
Article in Scientific reports, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT06509776 (Early Life Determinants of Skeletal Maturation and Endocrine Health in Young Adults - A Nationwide Birth Cohort Study), which is not on this map. Cited by 44 papers, 2 of them syntheses that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Early Life Determinants of Skeletal Maturation and Endocrine Health in Young Adults - A Nationwide Birth Cohort Study
Who cites it
44 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Epigenetic Ageing and Breast Cancer Risk: A Systematic Review.Cancer medicine · 2024Pooled it
- A systematic review of childhood maltreatment and DNA methylation: candidate gene and epigenome-wide approaches.Translational psychiatry · 2021Pooled it
- DNA methylation-based surrogate markers of C-reactive protein and their associations with health-related traits.GeroScience · 2026Article
- Biological Versus Technical Reliability of Epigenetic Clocks and Implications for Disease Prognosis and Intervention Response.Aging cell · 2026Article
- Epigenetic Age and Resilience in the Framingham Heart Study.Neurology open access · 2026Article
- Integrative epigenomic landscape of Alzheimer's Disease brains reveals oligodendrocyte molecular perturbations associated with tau.Nature communications · 2026Article
- Social relationships and epigenetic markers of aging in middle-aged and older adults: cross-sectional and prospective analyses.The journals of gerontology. Series B, Psychological sciences and social sciences · 2025Article
- Article
- Psychological distress in older adults: associations with epigenetic markers of ageing, inflammation, and depression, and joint effects on mortality.Brain, behavior, & immunity - health · 2025Article
- Association of Epigenetic Markers of Aging With Prevalent and Incident Type 2 Diabetes.The journals of gerontology. Series A, Biological sciences and medical sciences · 2025Article
- Dietary factors and DNA methylation-based markers of ageing in 5310 middle-aged and older Australian adults.GeroScience · 2025Article
- Article
- Identification and functional characterisation of DNA methylation differences between East- and West-originating Finns.Epigenetics · 2024Article
- Self-rated health, epigenetic ageing, and long-term mortality in older Australians.GeroScience · 2024Article
- Epigenetic Aging and Racialized, Economic, and Environmental Injustice: NIMHD Social Epigenomics Program.JAMA network open · 2024Article
- Body Size, Diet Quality, and Epigenetic Aging: Cross-Sectional and Longitudinal Analyses.The journals of gerontology. Series A, Biological sciences and medical sciences · 2024Article
- Epigenetic aging & embodying injustice: USmedRxiv : the preprint server for health sciences · 2023Article
- Biological stability of DNA methylation measurements over varying intervals of time and in the presence of acute stress.Epigenetics · 2023Article
- A systematic evaluation of normalization methods and probe replicability using infinium EPIC methylation data.Clinical epigenetics · 2023Article
- Connections between cross-tissue and intra-tissue biomarkers of aging biology in older adults.Epigenetics communications · 2023Article
Corrections and comments
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Authors and funding
12 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The reliability of methylation measures from the widely used HumanMethylation450 (HM450K) microarray has not been assessed for DNA from dried blood spots (DBS) or peripheral blood mononuclear cells (PBMC), nor for combined data from different studies. Repeated HM450K methylation measures in DNA from DBS and PBMC samples were available from participants in six case-control studies nested within the Melbourne Collaborative Cohort Study. Reliability was assessed for individual CpGs by calculating the intraclass correlation coefficient (ICC) based on technical replicates (samples repeated in a single study; 126 PBMC, 136 DBS) and study duplicates (samples repeated across studies; 280 PBMC, 769 DBS) using mixed-effects models. Reliability based on technical replicates was moderate for PBMC (median ICC = 0.42), but lower for DBS (median ICC = 0.20). Study duplicates gave lower ICCs than technical replicates. CpGs that were either highly methylated or unmethylated generally had lower ICCs, which appeared to be mostly related to their lower variability. The ICCs for global methylation measures were high, typically greater than 0.70. The reliability of methylation measures determined by the HM450K microarray is wide-ranging and depends primarily on the variability in methylation at individual CpG sites. The power of association studies is low for a substantial proportion of CpGs in the HM450K assay.
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