ArticleMicrobial ecology2016
Metatranscriptome analysis of active microbial communities in produced water samples from the Marcellus Shale.
Article in Microbial ecology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Pathways for Potential Exposure to Onshore Oil and Gas Wastewater: What We Need to Know to Protect Human Health.GeoHealth · 2025Review
- Inhibition of Microbial Growth and Biofilm Formation in Pure and Mixed Bacterial Samples.Microorganisms · 2024Article
- Osmoregulation in freshwater anaerobic methane-oxidizing archaea under salt stress.The ISME journal · 2024Article
- New microbiological insights from the Bowland shale highlight heterogeneity of the hydraulically fractured shale microbiome.Environmental microbiome · 2023Article
- Enrichment of rare methanogenic Archaea shows their important ecological role in natural high-COFrontiers in microbiology · 2023Article
- Microbial communities in freshwater used for hydraulic fracturing are unable to withstand the high temperatures and pressures characteristic of fractured shales.Access microbiology · 2023Article
- Geochemistry and Multiomics Data Differentiate Streams in Pennsylvania Based on Unconventional Oil and Gas Activity.Microbiology spectrum · 2022Article
- The Microbial Community and Functional Potential in the Midland Basin Reveal a Community Dominated by Both Thiosulfate and Sulfate-Reducing Microorganisms.Microbiology spectrum · 2022Article
- The impact of bacterial diversity on resistance to biocides in oilfields.Scientific reports · 2021Article
- Integrated metagenomic and metatranscriptomic analyses of ultraviolet disinfection effects on antibiotic resistance genes and bacterial communities during wastewater treatment.Ecotoxicology (London, England) · 2021Article
- Molecular-level variation of dissolved organic matter and microbial structure of produced water during its early storage in Fuling shale gas field, China.Environmental science and pollution research international · 2021Article
- Taxonomic and Functional Shifts in the Sprout Spent Irrigation Water Microbiome in Response toApplied and environmental microbiology · 2021Article
- Metatranscriptomic Analysis of Oil-Exposed Seawater Bacterial Communities Archived by an Environmental Sample Processor (ESP).Microorganisms · 2020Article
- Identification of Persistent Sulfidogenic Bacteria in Shale Gas Produced Waters.Frontiers in microbiology · 2020Article
- Surface Water Microbial Community Response to the Biocide 2,2-Dibromo-3-Nitrilopropionamide, Used in Unconventional Oil and Gas Extraction.Applied and environmental microbiology · 2019Article
- Unconventional Oil and Gas Energy Systems: An Unidentified Hotspot of Antimicrobial Resistance?Frontiers in microbiology · 2019Article
- Micro-scale intermixing: a requisite for stable and synergistic co-establishment in a four-species biofilm.The ISME journal · 2018Article
- Bacterial Biomarkers of Marcellus Shale Activity in Pennsylvania.Frontiers in microbiology · 2018Article
- Predominance and Metabolic Potential of Halanaerobium spp. in Produced Water from Hydraulically Fractured Marcellus Shale Wells.Applied and environmental microbiology · 2017Article
- Draft Genome Sequence of Methanohalophilus mahii Strain DAL1 Reconstructed from a Hydraulic Fracturing-Produced Water Metagenome.Genome announcements · 2016Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Controlling microbial activity is a primary concern during the management of the large volumes of wastewater (produced water) generated during high-volume hydraulic fracturing. In this study we analyzed the transcriptional activity (metatranscriptomes) of three produced water samples from the Marcellus Shale. The goal of this study was to describe active metabolic pathways of industrial concern for produced water management and reuse, and to improve understanding of produced water microbial activity. Metatranscriptome analysis revealed active biofilm formation, sulfide production, and stress management mechanisms of the produced water microbial communities. Biofilm-formation and sulfate-reduction pathways were identified in all samples. Genes related to a diverse array of stress response mechanisms were also identified with implications for biocide efficacy. Additionally, active expression of a methanogenesis pathway was identified in a sample of produced water collected prior to holding pond storage. The active microbial community identified by metatranscriptome analysis was markedly different than the community composition as identified by 16S rRNA sequencing, highlighting the value of evaluating the active microbial fraction during assessments of produced water biofouling potential and evaluation of biocide application strategies. These results indicate biofouling and corrosive microbial processes are active in produced water and should be taken into consideration while designing produced water reuse strategies.
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