ArticleParasites & vectors2016
In vitro culture of Echinococcus multilocularis producing protoscoleces and mouse infection with the cultured vesicles.
Article in Parasites & vectors, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
23 citing papers in PubMed, 53 citations in OpenAlex.
- Repurposing niclosamide ethanolamine for alveolar echinococcosis reveals a disconnect between in vitro efficacy and in vivo outcome in the intraperitoneal infection model.PLoS neglected tropical diseases · 2026Article
- Crystal structure of Echinococcus multilocularis cystatin B reveals a novel feature in classical stefins.Scientific reports · 2026Article
- The genetic variation of mitochondrial sequences and pathological differences ofMicrobiology spectrum · 2025Article
- Induced hepatocyte-like cells derived from adipose-derived stem cells alleviates liver injury in mice infected with Echinococcus Multilocularis.Scientific reports · 2024Article
- Ultrasonography screening of hepatic cystic echinococcosis in sheep flocks used for evaluating control progress in a remote mountain area of Hejing County, Xinjiang.BMC veterinary research · 2024Article
- Immunological Characteristics of Hepatic Dendritic Cells in Patients and Mouse Model with LiverTropical medicine and infectious disease · 2024Article
- RNA sequencing reveals dynamic expression of genes related to innate immune responses in canine small intestinal epithelial cells induced byFrontiers in veterinary science · 2024Article
- Effects of Dihydroartemisinin against Cystic EchinococcosisInfection and immunity · 2023Article
- The Truman Show for Human Helminthic Parasites: A Review of Recent Advances in In Vitro Cultivation Platforms.Microorganisms · 2023Review
- A Diverse Virome Is Identified in Parasitic Flatworms of Domestic Animals in Xinjiang, China.Microbiology spectrum · 2023Article
- NIR-II fluorescence microscopic bioimaging for intrahepatic angiography and the early detection ofFrontiers in bioengineering and biotechnology · 2023Article
- Immunization withTropical medicine and infectious disease · 2022Article
- Suppressive effect of pseudolaric acid B onFrontiers in microbiology · 2022Article
- An Excretory Protein ofInfection and drug resistance · 2022Article
- Structural changes and expression of hepatic fibrosis-related proteins in coculture of Echinococcus multilocularis protoscoleces and human hepatic stellate cells.Parasites & vectors · 2021Article
- Molecular Characterization of a Tetraspanin TSP11 Gene inFrontiers in veterinary science · 2021Article
- Metformin Suppresses Development of the Echinococcus multilocularis Larval Stage by Targeting the TOR Pathway.Antimicrobial agents and chemotherapy · 2020Article
- Dual Role of Hepatic Macrophages in the Establishment of theFrontiers in immunology · 2020Article
- In vitro and in vivo Effects of Artesunate onDrug design, development and therapy · 2020Article
- Thioredoxin peroxidase secreted by Echinococcus granulosus (sensu stricto) promotes the alternative activation of macrophages via PI3K/AKT/mTOR pathway.Parasites & vectors · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundAlveolar echinococcosis (AE) is a lethal zoonosis caused by the fox-tapeworm Echinococcus multilocularis. The disease is difficult to treat and an effective therapeutic drug is urgently needed. Reliable models are essential for drug development. In this study, we developed both in vitro and in vivo models of larval E. multilocularis.
resultsThe protoscoleces (PSC) of E. multilocularis from jirds were successfully cultured in a modified RPMI1640 based medium containing 25 % (v/v) fetal bovine serum (FBS). After 100 days of culture, PSC developed to larval vesicles (small unilocular cysts) and the fast growing vesicles produced PSC in brood capsules. In addition, mice were intraperitoneally injected with 30 cultured small vesicles and 100 % of the mice had resulting metacestode masses.
conclusionsLarval protoscoleces and vesicles of E. multilocularis grow healthily in vitro in the RPMI1640 based medium containing 25 % FBS. Echinococcus multilocularis in vitro and in vivo models provide a valuable platform for investigating the biology of the parasite and screening effective therapeutic drugs against AE.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.